T CELL REGULATORY MECHANISMS IN PERIODONTAL DISEASE

先前在人类和实验动物中的研究 提供了证据表明，抗原特异性T细胞发挥调节作用， 牙周病的危害 然而， 关于涉及T细胞亚群的性质， 调节口腔健康或炎症和牙周骨丢失， 也就是说，牙周病 建议的总体目标 研究的目的是研究T细胞的调节机制， 参与局部组织炎症和随后的骨质流失 与牙周病有关。 这些研究将采用 实验大鼠模型，并将具体涉及隔离， T细胞克隆的产生和表征， 特别有助于对牙周病原体类杆菌的反应 牙龈炎 这些克隆的T辅助细胞（Th）的能力， 调节同种型特异性B细胞生长和分化， 产生介导组织破坏和修复的因子， 被评价。 此外，抗原呈递的影响 细胞在介导T细胞效应中的作用，以及这些克隆细胞的能力， Th细胞对实验性大鼠牙周骨丢失的影响 模型将被确定。

Previous studies in humans and experimental animals have provided evidence that antigen-specific T cells play a regulatory role in periodontal disease. However, little is still known regarding the properties to the T cell subpopulation(s) involved in regulating oral health or inflammation and periodontal bone loss, i.e., periodontal disease. The overall objective of the proposed reseach is to investigate T cell regulatory mechanisms which may be involved in local tissue inflammation and subsequent bone loss associated with periodontal disease. The studies will employ an experimental rat model and will specifically involve the isolation, generation and characterization of T cell clones which specifically help responses to the periodontopathogen Bacteroides gingivalis. The ability of these cloned T helper (Th) cells to regulate isotype-specific B cell growth and differentiation and to produce factors which mediate tissue destruction and repair will be evaluated. In addition, the influence of antigen-presenting cells in mediating T cell effects, and the ability of these cloned Th cells to influence periodontal bone loss in an experimental rat model will be determined.