NHLBI CLINICAL INVESTIGATOR AWARD PROGRAM

免疫介导的血小板破坏发生在特发性(免疫) 血小板减少性紫癜(ITP)，过渡后紫癜(PTP)，新生儿 等免疫性血小板减少症(NITP)，以及其他疾病状态。这个 在这些情况下涉及的抗原和抗体不是很好 特色化的。使用我们开发的识别抗原的技术 承载着血小板的蛋白质，我们将研究其性质和结构。 这些血小板抗原和介导血小板的抗体 毁灭。这项技术涉及到血小板的电泳法分离。 膜蛋白，转移到硝酸纤维素纸上，接触抗体， 以及使用放射性标记的第二抗体检测抗体结合。我们 建议进一步鉴定PLA1血小板抗原，并对其进行研究。 NITP中抗PLA1抗体的性质。我们会尝试找出 与血小板相关的血小板Fc受体或Fab结合部位 免疫球蛋白可能会结合。最后，我们将进一步表征这些抗原和 我们在一名ITP儿童的受试者中发现了抗体。

Immune mediated platelet destruction occurs in idiopathic (immune) thrombocytopenia purpura (ITP), post transition purpura (PTP), neonatal isoimmune thrombocytopenia (NITP), and in other disease states. The antigens and antibodies involved in these conditions are not well characterized. Using techniques we have developed for identifying antigen bearing proteins of platelets, we will study the nature and structure of these platelet antigens and the antibodies which mediate platelet destruction. The technique involves electrophoretic separation of platelet membrane proteins, transfer to nitrocellulose paper, exposure to antibody, and detection of antibody binding using a radiolabeled second antibody. We propose to further characterize the PLA1 platelet antigen and to study the nature of the anti PLA1 antibodies in NITP. We will attempt to identify the platelet Fc receptor or Fab binding site to which platelet associated IgG may bind. Finally, we will further characterize the antigens and antibodies we have identified in a subject of children with ITP.