CELL SURFACE IN ADHERENCE OF BACTEROIDES GINGIVALIS

虽然牙龈类杆菌似乎在牙周炎中起着核心作用 与牙周炎相关的复杂微生物相互作用， 基本上没有数据表明这是如何 微生物在易感宿主中建立自己 最终参与疾病的进程。细菌黏附 是建立许多东道主的先决条件- 相关微生物及其随后的毒力。 虽然牙龈假单胞菌附着在某些其他微生物上， 这种类型的互动在建立中的潜在作用 而这种有机体的出现在很大程度上仍未被探索。这 研究将检查细菌间的粘附性。 具有选定的龈下微生物区系成员的牙龈， 并将尝试鉴定和表征细胞表面 具有潜在重要性的分子(即粘附素) 这种微生物的出现。医师-科学家奖 将为首席调查员提供教化教育 (第一阶段)和实验室培训(第一阶段和第二阶段) 关于复杂生物相互作用的研究。重要的是，在 本培训计划的两个阶段，它将暴露校长 个人和部门的调查员，他们的研究 人们感兴趣的是分子发病机制。这项研究建议 将检查牙龈假单胞菌对选定菌株的粘附性 已知存在于牙周的微生物 牙龈假单胞菌出现前的牙周袋 使用共同聚集技术(具体目标1)。表面 可能与牙龈假单胞菌黏附有关的结构 从生化和免疫学角度分离和鉴定 (具体目标2)。对具体问题的全面审查 牙龈假单胞菌对一个或多个测试菌株的粘附性将是 用来描绘这些表面分子的功能 粘附素(特定目标3)。粘附性牙周炎杆菌(S) 进一步以重组DNA技术为特征 描述或分离负责它们产生的基因(S) (具体目标4)。这是这项研究的长期目标(但 超出本裁决的范围)来审查 牙周炎活体模型中的细菌间黏附， 可能导致制定干预战略 随着这种微生物在牙龈下的建立 环境，并可能干扰疾病的发展。

While Bacteroides gingivalis appears to play a central role in the complex microbial interactions associated with periodontitis, there are essentially no data which indicate how this microorganism establishes itself in a susceptible host to eventually participate in the disease process. Bacterial adherence is a prerequisite for both the establishment of many host- associated microorganisms and their subsequent virulence. Although B. gingivalis adheres to certain other microorganisms, the potential role of this type of interaction in the establishment and emergence of this organism remains largely unexplored. This research will examine the interbacterial adherence of B. gingivalis with selected members of the subgingival microbiota, and will attempt to identify and characterize cell surface molecules (i.e., adhesins) of potential importance in the emergence of this microorganism. The Physician-Scientist Award will provide the Principal Investigator with the didactic education (Phase I) and laboratory training (Phase I and II) required for studies on the complex biological interactions. Importantly, in both phases of this training program, it will expose the Principal Investigator to individuals and departments whose research interests are in molecular pathogenesis. The research proposed will examine the adherence of B. gingivalis to selected periodontal microorganisms which are known to be established in the peroidontal pocket prior to the emergence of B. gingivalis with a co-aggregation technique (Specific Aim 1). Surface structures potentially involved in B. gingivalis adherence will be isolated and characterized biochemically and immunologically (Specific Aim 2). A comprehensive examination of the specific adherence of B. gingivalis to one or more test strains will be carried out to delineate those surface molecules functioning as adhesins (Specific Aim 3). The B. gingivalis adhesin(s) will be further characterized by recombinant DNA technology to delineate or isolate the gene(s) responsible for their production (Specific Aim 4). It is the long range goal of this research (but beyond the scope of this award) to examine the role of interbacterial adherence in in vivo models of periodontitis, leading potentially to the development of strategies to interfere with the establishment of this microorganism in the subgingival environment, and possibly to interfere with disease development.