ROLE OF THROMPOSPONDIN IN ODONTOGENESIS

该奖项的目的是为申请人提供机会， 获得必要的技能，成为一个完全独立的生物医学 具有分子生物学应用专门知识的调查员 上皮间质细胞病理学问题的技术 交互.有了这些技能，申请人将应用他的培训 研究颅面发育和口腔病理学， 发展牙科学术事业奖项的初始阶段 将在跨学科课程的教学研究中度过， 密歇根大学的细胞和分子生物学 实验室轮换学习不同的分子生物学技术 (RFLP分析，转基因小鼠程序，克隆技术，位点 定向诱变，哺乳动物细胞中的cDNA表达，原位 胚胎学中靶蛋白杂交、免疫定位 组织）。最后阶段将包括这些研究的应用 研究细胞外细胞粘附蛋白的作用的工具 （血小板反应蛋白）在牙形成中的作用。本研究的目的是整合 血小板反应蛋白的作用，以迅速扩大知识， 细胞粘附蛋白，其他细胞外基质蛋白，和细胞粘附蛋白。 上皮-间质相互作用中的跨膜受体 发生在牙齿形成中。由于牙齿的形成是一个模型，以研究 基因调控器官时空形成的方式 关于不同胚胎细胞系的相互作用， 从这个模型应该适用于发展（正常和 其他器官系统的异常。

The purpose of this award is to provide the applicant with the opportunity to obtain the skills necessary to become a fully independent biomedical investigator with expertise in the application of molecular biological techniques to questions of the pathology of epithelial-mesenchymal interactions. With these skills, the applicant will apply his training towards research in craniofacial development and oral pathology while developing a career in academic dentistry. The initial phase of the award will be spent in didactic study within the interdisciplinary program in Cellular and Molecular Biology at the University of Michigan and in laboratory rotations learning different techniques of molecular biology (RFLP analysis, transgeneic mouse procedures, cloning technology, site directed mutagenesis, cDNA expression in mammalian cells, in-situ hybridization, immunolocalization of target proteins in embryologic tissues). The final phase will consist of application of these research tools to investigate the role of an extracellular, cell adhesion protein (thrombospondin) in odontogenesis. The aim of this research is to integrate the role of thrombospondin to the rapidly expanding knowledge available on cell adhesion proteins, other extracellular matrix proteins, and cellular transmembrane receptors in the epithelial-mesenchymal interactions occurring in tooth formation. Since tooth formation is a model to study the way genes regulate the spatial and temporal formation of organs dependent on the interaction of different embryonic cell lines, information obtained from this model should be applicable to the development (normal and aberrant) of other organ systems.