DEVELOP LIVE ATTENUATED COLD-ADAPTED INFLUENZA VACCINE

提供甲型和乙型流感病毒冷适应重组疫苗 与相关的表面抗原一起用于英曼。找出一个稳定的、高的 产生供体菌株，用于生产B型重组疫苗。 A型和B型CA供体病毒重组体的获得和鉴定 来自这些CA患者的单基因。野战疫苗的测试分离株 对生物标记和随后的基因类型中的异常进行试验。 提供A型和B型供体的重组DNA克隆并测序 基因。提供使用二价和多价的基准数据 动物模型中应用于人类的疫苗制剂。这个 旨在建立感冒的遗传机制的方法 适应和衰减。实现这些目标所需的努力 目标的实现将符合以下指导方针的成本效益 这些疫苗的最终许可。

Provide cold-adapted reassortant vaccines of influenza virus types A and B with the relevant surface antigens for use inman. Identify a stable, high yielding donor strain for use in producing type B reassortant vaccines. Derive and characterize reassortants of both type A and B ca donor viruses with single genes from these ca patients. Test isolatesfrom field vaccine trials for anomalies in biological markers and subsequently in genotypes. Provide and sequence recombinant DNA clones of both types A and B donor genes. Furnish baseline data from the use of bivalent and polyvalentca vaccine preparations in an animal model for application in man. The approaches are designed to establish the geneticmechanisms of cold adaptation and attenuation. The efforts necessary to achieve these objectives will be performed withcost effectiveness along guidelines for the eventual licensing of these vaccines.