MORPHOLOGY AND FUNCTION OF THE BASAL GANGLIA

该计划的目标是研究功能和 使用各种技术开发基底神经节， 准备工作 该提案的力量来自于 在六个实验室中建立了工作，其主要负责人 调查人员拥有代表各个领域的不同背景， 解剖学、电生理学、药理学、分子和细胞 生物学和行为学。 这些调查人员由一个 共同的原因;即了解功能和 基底神经节的发展，根据多学科和 高度互动的研究项目。 我们计划调查a） 突触的发育、顺序和地形 新纹状体，B）递质和形态学的发育 纹状体神经元的表型和 它们的离子电导，c）假定的递质对 基底神经节神经元，d）鉴定新的推定的 苍白球中的神经递质，e）单个单位活动 在清醒动物的基底神经节中，和f）在清醒动物的基底神经节中的脑移植物， 基底神经节 项目1旨在描述主要的分离 大鼠组织块培养神经递质的表达， 免疫细胞化学形态表型 结合光学和电子显微镜，离子 全细胞和单通道电压钳电导 技术，以及多巴胺对这些神经元的电生理作用。 神经元 项目2将研究多巴胺的作用， 乙酰胆碱在活体大鼠纹状体脑片制备中的作用 细胞内记录技术与形态学关系 多巴胺传入和纹状体神经元之间的联系 项目3研究 纹状体神经元的放电模式在执行一个 灵长类动物的感觉触发运动任务。 伴随 解剖学研究，使用免疫细胞化学示踪结合 提出了用光学显微镜和电子显微镜观察的方法。 项目4将 确定正常细胞连接的程度 排列在纹状体移植物中通过细胞内 结合光的记录和PHA-L追踪方法 和电子显微镜。

The goal of this program is to investigate the function and development of the basal ganglia using a variety of techniques and preparations. The strength of the proposal has been built from established work in each of six laboratories, whose principal investigators possess diverse backgrounds representing the fields of anatomy, electrophysiology, pharmacology, molecular and cell biology, and behavior. These investigators are united by a common cause; namely to understand the function and development of the basal ganglia under a multidisciplinary and highly interactive research program. We plan to investigate a) development of synapses and order and topography in the neostriatum, b) development of transmitter and morphological phenotype of the striatal neurons and the characterization of their ionic conductances, c) action of putative transmitters on the basal ganglia neurons, d) identification of new putative neurotransmitters in the globus pallidum, e) single-unit activities in the basal ganglia of awake animals, and f) brain graft in the basal ganglia. Project 1 will intend to characterize in the primary dissociated rat explant culture the expression of neurotransmitter and morphological phenotypes using immunocytochemical techniques in conjunction with light and electronmicroscopy, ionic conductances by whole-cell and single-channel voltage clamp techniques, and electrophysiological effects of dopamine on these neurons. Project 2 will examine the action of dopamine and acetylcholine in in vivo rats striatal slice preparation by the intracellular recording technique and morphological relationship between dopamine afferents and striatal neurons. Project 3 study the firing pattern of striatal neurons during the performance of a sensory-triggered movement tasks in the primate. Concomitant anatomical studies, using immunocytochemical tracing combined with light and electronmicroscope are proposed. Project 4 will determine the extent to which the normal cellular connectional arrangements are reproduced in striatal graft by intracellular recording and PHA-L tracing methods in conjunction with light and electron microscopy.