PROPOSED NATIONAL VIBRATING PROBE FACILITY

我们建议在海洋生物研究所建立一个国家设施， 实验室（MBL）包含先进的仪器， 超灵敏振动探头 这将使各成员的 生物医学界有效地绘制和测量电场 各种生活系统;从而确定模式和（部分） 穿越这些系统的离子电流的性质。 第一 安装将包含两个章动探头系统， 相关的视频和计算机系统，以有效地减少，显示， 记录测量的场。 摆动探头提供同时 在任何一点上测量场的两个分量，并应允许 有效探索了穿越海洋的稳定海流， 在微小的细胞突起如神经突周围的水性介质以及 更大的细胞和组织，如卵子和肌肉。 在适当的时候，我们 建议开发振动探头的进一步版本，包括 测量空中系统（如某些昆虫）附近田地的空中测量器 蛋、真菌、哺乳动物皮肤等），一个三维的。 我们计划 开展各种合作研究项目，特别是关于 内源性海流在某些海洋中形成模式作用 卵和极化哺乳动物卵裂球，以及内源性 尖端电流控制神经生长。 我们希望满足 各种各样的生物医学用户，包括感兴趣的人， 通过肌肉、神经和血细胞的内源性电流，通过各种 卵子和胚胎，通过生长植物细胞， 上皮细胞、再生肢体和神经残端等等。 我们同样 计划提供有关振动探头使用的适当培训。

We propose to establish a national facility at the Marine Biological Laboratory (MBL) containing advanced instrumentation based upon the ultrasensitive vibrating probe. This will enable various members of the biomedical community to efficiently map and measure the electrical fields around various living systems; and thus determine the pattern and (in part) the nature of the ionic currents which traverse these systems. The first installation will contain two nutating probe systems together with associated video and computer systems to efficiently reduce, display, and record the fields which are measured. Nutating probes provide simultaneous measurements of two components of the field at any point and should allow the effective exploration of the steady currents which traverse the acqueous media around minute cellular processes such as neurites as well as larger cells and tissues such as eggs and muscles. In due course we propose to develop further versions of the vibrating probe including an aerial one to measure fields near aerial systems (such as certain insect eggs, fungi, mammalian skin, etc.), and a three dimensional one. We plan to carry out various collaborative research projects, particularly ones on the role of endogenous currents in establishing pattern in certain marine eggs and in polarizing mammalian blastomeres, and the role of endogenous tip currents in controlling nerve growth. We expect to serve the needs of a very wide variety of biomedical users including persons interested in endogenous currents through muscle, nerve and blood cells, through various eggs and embryos, through growing plant cells, various functioning epithelia, regenerating limbs and nerve stumps and so forth. We likewise plan to provide appropriate training in the use of the vibrating probe.