NIH/MSU MASS SPECTROMETRY FACILITY

该应用程序需要资金，以便继续为研究服务 具有艺术方面技术专长的NIH受赠者的利益， 质谱法的实践。 核心研究将集中在设计上， 时间阵列检测（TimeArrayDetection，简称MIMO）实现及其应用。 一个主要 将在飞行时间（TOF）质谱法中应用， 可以检测和积分所有的离子电流（在整个 质量范围）从离子源加速。 预计这种能力 使仪器的全光谱灵敏度增加至少 的数量级，并增加了获取可用光谱的速率 数量级。 这两个特点将对 传统的GC-MS，特别是越来越强调使用 毛细管柱在复杂混合物的分析中发现， 生物样本 在另一个核心项目中， 安装在磁性仪器（LKB-9000）上，以允许组合 质量的磁场分散和时间分辨率， 区分“亚稳峰”和进行“MS/MS”分析 快速地在单聚焦质谱仪上进行。合作研究 将强调有机代谢分析方法的扩展 尿液、血浆和/或羊水中的酸，用于研究改变的 代谢在人类疾病状态，如糖尿病和肌肉 营养不良 异生物质改变与甾体代谢异常 将研究大鼠中的混合功能氧化酶。 特征分析方法 还将用于表征感染性疾病的代谢途径， 寄生虫的观点，以发展一个合理的基础，纠正 和预防性药物治疗。 对服务和合作的要求将 要求分析生理生化样品中的芦荟素， 糖、吲哚衍生物等。许多项目涉及有机合成 将继续要求精确的质量测量，以验证经验 其产品的配方。 培训将继续是重要的 在设施计划的功能;大约20名研究生收到 通过研究项目，与 五名专业调查员

This application reuests funds in order to continue to serve the research interests of NIH grantees with technical expertise in the arts and practices of mass spectrometry. Core research will focus on the design, implementation, and application of time array detection (TAD). A major application of TAD will be in time-of-flight (TOF) massspectrometry to make it possible to detect and integrate all the ion current (over the complete mass range) accelerated from the ion source. This capability is expected to increase the full spectrum sensitivity of the instrument by at least an order of magnitude and increase the rate of acquiring useable spectra by an order of magnitude. Both of these features will have a profound effect on conventional GC-MS, especially with increasing emphasis on the use of capillary columns in the analysis of complex mixtures found in most biological samples. In another core project, a device for TAD will be installed on a magnetic instrument (LKB-9000) to permit a combination of magnetic field dispersion and time resolution of mass to be employed for distinguishing "metastable peaks" and for performing "MS/MS" analyses rapidly on a single focussing mass spectrometer. Collaborative reserach will emphasize extension of metabolic profiling methodology for organic acids in urine, plasma, and/or amniotic fluid for investigations of altered metabolism in human disease states such as diabetes and muscular dystrophy. Aberrant steriod metabolism related to xenobiotic alteration of mixed function oxidases in rats will be studied. Profiling methodology will also be used to characterize metabolic pathways in infectious parasites with the view toward developing a rational basis for corrective and prophylactic drug therapy. Requests for service and collaboration will call for analyses of physiological biochemical samples for prostaglandins, sugars, indole derivatives, etc. Many projects involving organic synthesis will continue to request exact mass measurements to validate the empirical formulas of their products. Training will continue to be an important feature in the Facility program; approximately 20 graduate students receive detailed training in mass spectrometry through research projects with the five professional investigators in the Facility program.