INTEGRATED MICROSCOPY FACILITY FOR BIOMEDICAL RESEARCH

高压电子显微镜设施是美国的生物技术资源 美国国立卫生研究院生物技术资源分支。并可用于生物医学 研究人员进行超微结构研究，其中使用高压电子 显微镜可以提供新的信息。 调查特别针对 对于分析相对较大和复杂的三维细胞 完整细胞或分离的细胞器中的结构，如分离的 鞭毛、染色体和高尔基复合体，或在长达1或2微米的切片中 厚 冷级将进行进一步的开发和改造。 冷冻和冷样品处理程序已开发，以供查看 冷冻切片、完整标本、分离的细胞成分和水溶液 暂停。 一种改进型像增强器和数字图像存储器 处理器已安装并成功使用。 这是一个项目的一部分 提高高倍率下的分辨率和性能， 可能的高分子晶体的高分辨率研究。 H.V.E.M.是 特别适合这种类型的高分辨率研究，因为它 更大的穿透力，更大的景深， 样品室尺寸，一个足够大，以允许引入 辅助设备，如倾斜/冷却台，其是必要的， 保护和定位这些生物结构。 正在制定方法 用于细胞结构如细胞骨架的免疫细胞化学标记 元件，无论是在细胞整体安装和厚部分。

The High Voltage Electron Microscope Facility is a Biotechnology Resource of the Biotechnology Resources Branch of N.I.H. and is available to bio-medical researchers for ultrastructural studies where use of a high voltage electron microscope can provide new information. Investigations are directed especially towards analysis of relatively large and complex three-dimensional cell structures either in intact cells or isolated organelles, such as isolated flagella, chromosomes and Golgi complexes, or in sections up to 1 or 2 microns thick. The cold stage will undergo further development and modification. Freezing and cold sample handling procedures have been developed for viewing frozen sections, whole specimens, isolated cell components and aqueous suspensions. An improved type of image intensifier and digital image/memory processor has been installed and successfully used. This is part of a program to improve resolution and performance at high magnification in order to make possible high resolution studies of macromolecular crystals. The H.V.E.M. is particularly well suited to high resolution studies of this type because of its greater penetrating power, its greater depth of field and because of its greater specimen chamber size, one sufficiently large to permit introduction of ancillary equipment such a tilting/cooling stages which are necessary to preserve and orient these biological structures. Methods are being developed for immunocytochemical labeling of cell structures such as cytoskeletal elements, both in cell whole mounts and in thick sections.