CORE--TISSUE CULTURE, MONOCLONAL ANTIBODY, HISTOPATHOLOGY, AND FLOW CYTOMETRY

该中心赠款的大多数项目已经并将继续依赖于 培养的和分离的滑膜的可用性不同 微环境成分以及新鲜和可获得的 培养的滑膜T细胞制剂。核心设施提供了 所有这些组织的中央来源，并应用了特殊的 分离和生长滑膜衬里细胞所需的专业知识。 每个人体组织或外周血液样本都被登录、处理、 在组织的情况下，为以后的培养和为了 免疫组织学，为光学显微镜准备的部分，和部分 为电子显微镜做准备。单抗的制备及应用 该中心的许多调查人员和核心组织都需要抗体 INFILE是众多单抗的中心来源。 免疫荧光染色组织切片和原位组织切片 杂交分析对许多研究都是必要的，并且仍然存在。 对于滑膜炎症的持续研究是必不可少的。一种新的 除了核心设施外，还提供了流式细胞仪设施 和用于淋巴表型分析和分离的专业知识 细胞和培养的滑膜衬里细胞或唾液上皮细胞。这个 这个核心设施的主要理由来自于高度 滑膜生长和特征化的劳动密集性 微环境组成部分和消除重复劳动 个人研究人员在获得必要的细胞种群和 研究用的纸巾。这个核心单位I的具体目标是培养 并提供新鲜的巨噬细胞和T细胞样本 研究人员进行功能研究，以维持滑膜组织库 提供新鲜或冷冻的滑膜组织样本以供研究，以提供 组织切片给进行原位杂交研究的研究人员， 免疫荧光分析，或光学显微镜分析，以提供 用于研究的单抗，并提供流式细胞仪设备 以及调查人员的专业知识。这个设施被设计成一个 组织培养细胞和组织切片的重要来源 对中心的所有成员开放。

Most projects of this center grant have depended and continue to depend to varying on the availability of cultured and isolated synovial microenvironment components as well as the availability of fresh and cultured synovial T cell preparations. The Core Facility provides a central source for all of these tissues and has applied the special expertise necessary to isolation and growth of the synovial lining cells. Each human tissue or peripheral blood specimen is logged in, processed, and, in the case of tissue, portions frozen for later culture and for immunohistology, portions prepared for light microscopy, and portions prepared for electron microscopy. The preparation and use of monoclonal antibodies are required by many of the Center's investigators and the Core Facility serves as a central source of numerous monoclonal antibodies. Tissue sections for immunofluorescence staining and for in situ hybridization assays have been necessary to many studies and remain essential to the continuing studies of synovial inflammation. A new addition to the Core Facility is the provision of flow cytometry facilities and expertise in use for phenotypic analysis and separation of lymphoid cells and cultured synovial lining cells or salivary epithelial cells. The principal justifications for this Core facility derive from the highly labor intensive nature of growing and characterizing synovial microenvironment components and the elimination of duplication of effort by individual investigators in obtaining the necessary cell populations and tissues for study. The specific aims of this Core Unit I are to culture synovial cells and to provide fresh samples of macrophages and T cells to investigators for functional studies, to maintain a synovial tissue bank to provide fresh or frozen synovial tissue samples for studies, to provide tissue sections to investigators performing in situ hybridization studies, immunofluorescent assays, or light microscopic analysis, to provide monoclonal antibodies for studies, and to provide flow cytometry facilities and expertise to investigators. This facility is designed to be an important source of tissue culture cells and tissue sections that will be available to all members of the Center.