THE ROLE OF GROWTH HORMONE IN THE AGING PROCESS

我们的假设是，生长激素缺乏可能是负责一些 与衰老相关的生理变化，包括矿物质的损失， 骨骼、皮肤弹性降低和瘦体重减少。 该项目的具体目标是：（1）确定 生长调节素-C（Sm-C）和平均积分显著降低 血清GH水平（ICGH）的浓度发生在正常男性中， 女性 (2)确定50岁以上低血糖的男性 ICGH和Sm-C血浆水平从垂体释放GH时， 药物刺激，导致生长激素释放的儿童和年轻人。 (3)确定与N2和Ca相关的急性代谢反应 平衡，脂质和脂蛋白浓度，葡萄糖耐量， 羟脯氨酸排泄，和Sm-C浓度，当GH用于 5-7正常男性（大于45岁），GH和Sm-C低 水平，与GH缺乏的年轻人的反应相比。 (4)确定每日GH给药3年对 20-30岁GH缺乏受试者（HA组）的身体组成， 对45岁以上正常男性身体成分的影响（组 O）。 HA组和O组将分为2组（HA 1和HA 2中10个，HA 2中15个）。 01和02）。 01和HA 1将每天接受GH。 02和HA 2将作为 对照 待评估的身体组成参数包括全身 N2、Ca、脂肪、瘦体重、骨密度、全身水分和皮肤 混合物. 将分析穿刺活检的皮肤类型， 通过SDS电泳测定的I型和III型胶原的量;纤连蛋白， 层粘连蛋白和IV型胶原的免疫荧光抗体技术; 通过AA分析的羟脯氨酸和羟赖氨酸水平：可还原交叉 用色谱法测定胶原中的连接.纤维束直径的测量 和定向;以及血浆中的纤连蛋白水平。 将在个体之间进行所有身体成分研究的比较 在组内和组间，以确定随着年龄的变化， 由于长期服用GH而引起的变化。 除了获得的科学知识，可以想象， 生长激素可使身体组织恢复到早期状态， 老年人的管理。

Our hypothesis is that GH deficiency may be responsible for some physiologic changes associated with aging, including loss of mineral from bone, decreased skin elasticity and loss of lean body mass. The specific aims of the project are: (1) To establish the age(s) at which a significant decrease in somatomedin-C (Sm-C) and mean integrated concentrations of serum GH levels (ICGH) occurs in normal males and females. (2) To determine if males Greater than 50 years of age with low ICGH & Sm-C levels in plasma release GH from the pituitary when given pharmacologic stimuli which cause GH release in children & young adults. (3) To determine the acute metabolic responses relating to N2 and Ca balance, lipid and lipoprotein concentrations, glucose tolerance, hydroxyproline excretion, and to Sm-C concentrations, when GH is given for 5-7 days to normal males (Greater than 45 years), with low GH and Sm-C levels, as compared to the response of young adults who are GH deficient. (4) To establish the effect of daily GH administration for 3 years on the body composition of GH deficient subjects 20-30 years of age (Group HA) and on the body composition of normal males Greater than 45 years of age (Group O). Groups HA and O will be divided into 2 groups (10 in HA1 & HA2, 15 in 01 & 02). 01 & HA1 will receive GH each day. 02 & HA2 will serve as controls. Parameters of body composition to be assessed include total body N2, Ca, fat, lean body mass, bone density, total body water, and skin composition. Skin from punch biopsies will be analyzed for the type and quantity of Type I & III collagen by SDS electrophoresis; fibronectin, laminin, and Type IV collagen by immunofluorescent antibody techniques; levels of hydroxyproline and hydroxylysine by AA analysis: reducible cross links in collagen by chromatography; measurement of fiber bundle diameter and orientation by electron microscopy; and fibronectin levels in plasma. Comparison of all body composition studies will be made between individuals in groups and between groups to ascertain changes with aging and alterations as a result of long term GH administration. In addition to the scientific knowledge gained, it is conceivable that restoration of body tissues to an earlier state could occur with GH administration in older individuals.