Time-Resolved Emission Imaging Microscopy with long-lived Pt(II) complexes: a new approach to autofluorescence-free imaging of tissues

背景自体荧光的问题在复杂细胞和组织研究的许多领域中都是相当普遍的。这包括各种终端，其中荧光成像是绝对需要的，例如临床诊断、医学研究以及细胞和组织生物学的基础研究。我们的简短提案旨在通过使用一种名为时间分辨发射成像(TREM)的技术来解决这些问题--这是我们在2008年实现的一种方法。由于最近开发了非常稳定和长寿命的铂(II)发光络合物(也是我们开发的)，TREM的潜在价值才刚刚成为现实。然而，这些分子的真正可开发价值是通过与多种可能的免疫标记靶标的选择性高亲和力抗体结合而具有多功能性。对于传统上受到自发荧光阻碍的复杂组织的成像，这将具有重大的技术优势，因为它使蛋白质和细胞能够在没有荧光干扰的情况下被隔离分解，否则会阻碍详细的成像。

The problems of background autofluorescence are considerable and widespread in many avenues of complex cell and tissue research. This includes a diverse range of endpoints where fluorescence imaging is an absolute requirement, and examples include clinical diagnostics, medical research and the fundamental study of cell and tissue biology. Our short proposal aims to solve these problems by use of a technique called time-resolved emission imaging (TREM) - a method realised by us in 2008. The potential value of TREM has only just become a practical reality due to the recent development of very stable and long-lived Pt(II) luminescent complexes (also developed by us). However, the true exploitable value of these molecules is one of versatility by conjugation to selective high affinity antibodies for the multitude of possible immunolabelling targets. This will have a major technical advantage for imaging complex tissues traditionally hampered by autofluorescence, by enabling proteins and cells to be resolved in isolation without fluorescent interference that would otherwise prevent detailed imaging.