Time-Resolved Emission Imaging Microscopy with long-lived Pt(II) complexes: a new approach to autofluorescence-free imaging of tissues

采用长寿命 Pt(II) 配合物的时间分辨发射成像显微镜：组织无自发荧光成像的新方法

• 批准号: BB/G024235/1
• 负责人: JAG Williams
• 金额: $ 4.42万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG024235%2F1
• 关键词: Time; Resolved; Emission; Imaging; Microscopy

The problems of background autofluorescence are considerable and widespread in many avenues of complex cell and tissue research. This includes a diverse range of endpoints where fluorescence imaging is an absolute requirement, and examples include clinical diagnostics, medical research and the fundamental study of cell and tissue biology. Our short proposal aims to solve these problems by use of a technique called time-resolved emission imaging (TREM) - a method realised by us in 2008. The potential value of TREM has only just become a practical reality due to the recent development of very stable and long-lived Pt(II) luminescent complexes (also developed by us). However, the true exploitable value of these molecules is one of versatility by conjugation to selective high affinity antibodies for the multitude of possible immunolabelling targets. This will have a major technical advantage for imaging complex tissues traditionally hampered by autofluorescence, by enabling proteins and cells to be resolved in isolation without fluorescent interference that would otherwise prevent detailed imaging.

背景自发荧光的问题在复杂细胞和组织研究的许多途径中是相当重要和广泛的。这包括荧光成像是绝对要求的各种端点，例如临床诊断，医学研究以及细胞和组织生物学的基础研究。我们的简短建议旨在通过使用一种称为时间分辨发射成像（TREM）的技术来解决这些问题-这是我们在2008年实现的方法。由于最近开发了非常稳定和长寿命的Pt（II）发光配合物（也由我们开发），TREM的潜在价值才刚刚成为现实。然而，这些分子的真正可利用价值是通过与针对多种可能的免疫标记靶标的选择性高亲和力抗体缀合的多功能性。这对于传统上受自体荧光阻碍的复杂组织的成像将具有主要的技术优势，通过使蛋白质和细胞能够在没有荧光干扰的情况下分离，否则将阻止详细的成像。

项目成果

Lighting the way to see inside the live cell with luminescent transition metal complexes

• DOI: 10.1016/j.ccr.2012.03.018
• 发表时间: 2012-08-01
• 期刊: COORDINATION CHEMISTRY REVIEWS
• 影响因子: 20.6
• 作者: Baggaley, Elizabeth;Weinstein, Julia A.;Williams, J. A. Gareth
• 通讯作者: Williams, J. A. Gareth

Long-lived metal complexes open up microsecond lifetime imaging microscopy under multiphoton excitation: from FLIM to PLIM and beyond

• DOI: 10.1039/c3sc51875b
• 发表时间: 2014-03-01
• 期刊: CHEMICAL SCIENCE
• 影响因子: 8.4
• 作者: Baggaley, Elizabeth;Botchway, Stanley W.;Weinstein, Julia A.
• 通讯作者: Weinstein, Julia A.