Isolation of pooled selected gene segments for allele discovery by next generation sequencing

未来的作物品种将需要改善消费者和生产者的性能特征，并减少对环境的影响（例如，减少化肥或农药的投入）。这些特征目前由“分子标记”监测，这是一种基因指纹测试。另一种方法是直接对遗传信息进行排序。“下一代测序”的革命正在进行中，速度每年翻一番，价格暴跌，直接测序可以提供比分子标记高得多的准确性，但由于它在同一个容器中对许多个体进行测序，因此存在技术瓶颈，这引发了如何跟踪哪个序列属于哪个个体的难题。在这个项目中，我们建议开发、测试和比较两种有前途的跟踪方法，以大麦为试验台。

Future crop varieties will need improved performance traits for the consumer and the producer, and reduced environmental impact (e.g. lower fertiliser or pesticide input). These traits are currently monitored by 'molecular markers', which are genetic fingerprinting tests. An alternative approach is to sequence the genetic information directly. A revolution in 'next generation sequencing' is currently underway' with speed doubling annually and prices tumbling, Direct sequencing can give far greater accuracy than molecular markers but there is a technical bottleneck caused by the fact that it sequences many individuals in the same container, raising the difficult issue of how to keep track of which sequence belongs to which individual. In this project we propose to develop, test and compare two promising tracking methods, using barley as a test-bed.