ULTRASTRUCTURE AND FUNCTION OF NERVE AND MUSCLE

本项目的目的之一是研究 甲壳类动物的伸展感受器官与其功能的关系。 我们计划 研究感觉神经树突中拉伸引起的结构变化 神经元，其中发生器电位，通过使用超快速冷冻， 冷冻置换法。 此外，膜的精细结构， 受体神经元上的抑制性突触将通过使用 冷冻断裂法 该项目的另一个目标是调查 新形成的细胞的超微结构和电生理特性 组织培养中的神经肌肉接头。 我们还将研究 的连接外乙酰胆碱受体在培养的肌肉细胞， 微电生理学和冷冻断裂法。

One of the objectives of this project is to investigate the ultrastructure of the crustacean stretch receptor organ in relation to its function. We plan to investigate stretch-induced structural changes in the dendrities of the sensory neuron, where the generator potential occurs, by using ultraquick freezing and freeze-substitution methods. In addition, the membrane fine structure of the inhibitory synapses on the receptor neuron will be studied by using the freeze-fracture method. The other objective of this project is to investigate the ultrastructural and electrophysiological properties of newly-formed neuromuscular junctions in tissue culture. Also we will study the development of extrajunctional acetylcholine receptors in cultured muscle cells by using microelectrophysiological and freeze-fracture methods.