STUDIES OF FC RECEPTORS AND IG BINDING FACTORS

本研究的目的是确定Fc的结构和功能 淋巴细胞和单核/巨噬细胞上的IgE受体。1.小鼠淋巴细胞 对花粉过敏的患者将进行Fc ETA受体(Fc ETA)分析 R)一年期间、草花粉之前、期间和之后的阳性细胞 赛季到了。淋巴细胞将被培养，并在体外检测产生的IgE 使用未分离的和Fc ETA R耗尽或浓缩的细胞。2.单核细胞 将从过敏性疾病患者中提纯并研究Fc ETA R 阳性细胞。将测定IgE包被靶细胞的吞噬功能 细胞会导致过敏反应慢反应物质(SRS-A)从 单核细胞。3.将分析大鼠肺泡和腹膜巨噬细胞 它们与包被大鼠骨髓瘤蛋白的红细胞形成花环的能力 所有类和子类的。大鼠肺泡巨噬细胞有Fc 并将在与聚合体相互作用后进行SRS-A释放测试 骨髓瘤蛋白。4.Fc-ETA-R阳性淋巴细胞数量(均为T 和B细胞)和巨噬细胞将被检测感染的大鼠 巴西拟圆线虫寄生于各种淋巴器官。数字 这些细胞上的受体的数量将通过测量结合 用放射性标记的IgE标记分离的细胞。

The purpose of the research is to determine the structure and function of Fc receptors foR IgE on lymphocytes and monocytes/macrophages. 1. Lymphocytes of patients with allergies to pollen will be analyzed for Fc eta receptors (Fc eta R) positive cells over a year period, before, during and after the grass pollen season. The lymphocytes will be cultured and the IgE produced in vitro measured employing unfractionated and Fc eta R depleted or enriched cells. 2. Monocytes will be purified from patients with allergic disease and studied for Fc eta R positive cells. It will be determined if phagocytosis of IgE coated target cells will cause release of slow reacting substance of anaphylaxis (SRS-A) from monocytes. 3. Rat alveolar and peritoneal macrophages will be analyzed for their ability to form rosettes with red cells coated with rat myeloma proteins of all classes and subclasses. Rat alveolar macrophages were shown to have Fc eta R and will be tested for release of SRS-A after interaction with aggregated IgE myeloma proteins. 4. The numbers of Fc eta R positive lymphocytes (both T and B cells) and macrophages will be determined in rats infected with Nippostronglyus brasiliensis parasites in various lymphoid organs. The number of receptors on these cells will be quantitated by measuring binding of radiolabeled IgE to isolated cells.