HETEROGENEITY OF THYMUS DERIVED LYMPHOCYTES

自体混合淋巴细胞反应代表T细胞的活化 在没有常规抗原的情况下，细胞由自身Ia分子产生。 尽管在啮齿动物和人类身上已经描述了这种反应性，但它的 与免疫反应性发生所需的自我识别的关系 在活体内的情况尚不清楚。利用自体反应性人类T细胞克隆， 自体反应性人类T-T杂交体，一种出现的单抗 自身反应性T细胞所显示的Ia受体具有特异性 细胞，以及从自体反应性T细胞建立的cDNA文库 混合，将调查以下具体目标：1)确定 抗原反应性T细胞与自身反应性T细胞的关系 检测自体反应性T细胞促进 巨噬细胞的分化，3)检测阻断巨噬细胞 自体混合淋巴细胞反应，体内，对抗原反应性，和 4)描述自身Ia的T细胞受体，特别参考 以确定结构和基因的多态。这些研究并不是 唯一重要的是确立自我认识的重要性 以AMLR为代表，也是为了阐明T细胞的性质 用于自我Ia的识别单元。

The autologous mixed lymphocyte reaction represents the activation of T cells by self Ia molecules in the absence of conventional antigens. Although this reactivity has been described in rodents and humans, its relationship to self recognition required for immune reactivity occurring in vivo is not known. Utilizing autologous-reactive human T cell clones, autologous-reactive human T-T hybrids, a monoclonal antibody which appears to have specificity for the Ia receptor displayed by autologous reactive T cells, and a cDNA library established from an autologous reactive T cell hybrid, the following specific aims will be investigated: 1) determine the relationship between antigen reactive and autologous reactive T cells, 2) examine the ability of autologous reactive T cells to promote the differentiation of macrophages, 3) examine the effects of blocking the autologous mixed lymphocyte reaction, in vivo, on antigen reactivity, and 4) characterize the T cell receptor for self Ia with particular reference to determining structural and genetic polymorphism. These studies are not only important for establishing the significance of self recognition represented by the AMLR, but also for elucidating the nature of the T cell recognition unit for self Ia.