CELL SURFACE RECEPTORS ON CYTOTOXIC T CELLS

细胞毒性胸腺来源淋巴细胞(CTL)是胸腺细胞的重要组成部分。 免疫反应的效应器阶段。它在世界上发挥着核心作用 病毒感染细胞的破坏和异体组织的排斥 移植物或肿瘤。此外，CTL也可能参与了 某些自身免疫性疾病的发病机制。 这项建议的主要目的是研究靶抗原。 T细胞的识别和信号转导的初始阶段 感受器。拟议研究的目的是要解开 CTL表面T细胞受体/T_3复合体的复杂性及其意义 跨膜蛋白的生物合成及其可能的分子机制 配体/受体相互作用后的信号转导已经建立。在……里面 除了对人类T细胞受体的描述外，人类白细胞抗原A2和 -B7抗原，小鼠CTL的T细胞受体功能。 该系统具有许多独特的优势： 1)明确定义的人和小鼠细胞毒性T细胞克隆的可用性 和目标细胞。2)受体/抗原相互作用可以通过的分析 所研究的是常规使用的。3)有关蛋白质的大量信息 人和小鼠T细胞表面T细胞受体/T3复合体的结构 都被收集起来了。4)重组DNA技术方面的专业知识 是我们实验室引进的。 拟议项目的具体目标包括： A)T细胞表面受体B链和A链的研究 人类CTL，b)T细胞成分作用的研究 受体/T3复合体在细胞溶解的早期阶段，c)研究 α2-微球蛋白的作用及其辅助结构T8(Lyt2)和 LFA-1在CTL识别I类MHC抗原中的作用，d)详细 人和小鼠T细胞受体/T3组分的描述 络合物及其生物合成和，e)信号模式的检查 T细胞受体/T3复合体的转导。

The cytotoxic thymus derived lymphocyte (CTL) is a critical element in the effector phase of the immune response. It plays a central role in the destruction of virus-infected cells and in the rejection of foreign tissue grafts or tumors. Moreover, CTL's might also be involved in the pathogenesis of some autoimmune diseases. The main objective of this proposal is to study the target antigen recognition and the initial phase of signal transduction by T cell receptors. The intention of the proposed studies is to unravel the complexity of the T cell receptor/T3 complex on the surface of CTL, its biosynthesis and the possible molecular mechanisms of transmembrane signalling after ligand/receptor interaction has been established. In addition to a description of human T cell receptors specific for HLA-A2 and -B7 antigens, T cell receptor function of murine CTL will be studied. This system offers a number of unique advantages: 1) availability of well defined human and murine cytotoxic T cell clones and target cells. 2) assays by which receptor/antigen interactions can be studied are used routinely. 3) considerable information about the protein structure of the T cell receptor/T3 complex on human and murine T cells has been collected. 4) expertise in recombinant DNA technology has been introduced in our laboratory. The specific aims of the proposed project include: a) the study of the B and a-chains of the T cell receptor on the surface of human CTL's, b) the study of the role of the components of the T cell receptor/T3 complex in the early stages of cytolysis, c) investigation of the role of a2-microglobulin and the acessory structures T8 (Lyt2) and LFA-1 in recognition of class I MHC antigens by CTL, d) a detailed description of the components of the human and murine T cell receptor/T3 complex and its biosynthesis and, e) examination of the mode of signal transduction by the T cell receptor/T3 complex.