DELETION MAPPING OF HUMAN CELL SURFACE ANTIGENS

本研究的目的是：1)确定 人类白细胞抗原D区基因及补体成分B(F)和C4；2) 定位编码T细胞特异性抗原的基因，包括推测的T细胞 细胞抗原受体和编码细胞表面抗原的基因 β2-微球蛋白基因；3)鉴定和定位编码微球蛋白的基因 这些区域以前未绘制的或未检测到的抗原。整体而言 方法将是分离影响细胞表面的缺失变体 T、B淋巴系抗原的诱变和免疫选择 用适当的抗体。使用克隆的cDNA和可用的单抗 抗体，以及我们实验室生产的单抗，我们 将使用祖细胞及其同源细胞上的反应性模式 将基因映射到缺失区域的缺失变体，使用 在最初的赠款期间制定的分析方法。

The objectives of this research are: 1) to determine the order of the HLA-D genes and complement components B(f) and C4 in the HLA-D region; 2) to map genes coding for T-cell specific antigens, including the putative T cell antigen receptor and genes coding for cell surface antigens linked to the Beta2-microglobulin gene; and 3) to identify and map genes coding for previously unmapped or undetected antigens in these regions. The overall approach will be to isolate deletion variants affecting cell surface antigens of T and B lymphoid cell lines by mutagenesis and immunoselection with appropriate antibodies. Using cloned cDNAs and available monoclonal antibodies, as well as monoclonal antibodies produced in our laboratory, we will use the patterns of reactivity on progenitor cells and their congenic deletion variants to map the genes to the deleted regions, employing methods of analysis worked out in the initial grant period.