MURINE B LYMPHOCYTE DEVELOPMENT

免疫系统在个体发育过程中是如何发育的还不清楚 成为相互作用的T细胞和B细胞的连接网络。 的 这个正在进行的研究项目的长期目标是解决这个问题 使用T15/PC小鼠系统作为模型的问题。 我们 工作假设是，存在有限的资源分配给 个体发育过程中的B细胞分化。 因此，B细胞 先驱者与每一个竞争，一些成为赢家 （显性遗传型）。 T15+ B细胞克隆具有个体发育学特征， 优势和竞争成功，以达到发展 发生IghC相关网络选择以保留 这种独特型的显性表达。 本提案涉及 旨在重建细胞相互作用的实验， 带来T15的优势 我提出了一系列的体内 实验分析抗原的作用，PC的内部图像， 辅助性T和B细胞亚群在调节T15表达中的作用。 我们的研究解决了重要和悬而未决的问题， 免疫调节：独特型与非独特型特异性效应， 淋巴细胞网络的发展和相互依赖性 T和B细胞库。

It is not known how the immune system develops during ontogeny to become a connected network of interacting T and B cells. The long-term goal of this on-going research project is to solve this problem using the T15/PC murine system as a model. Our working hypothesis is that there exist finite resources allocated to B cell differentiation during ontogeny. As a consequence, B cell precursors compete with each and some become winners (dominant idiotypes). T15+ B cell clones have an ontogenetic advantage and compete successfully to reach a developmental stage where IghC-linked network selection occurs to preserve dominant expression of this idiotype. This proposal deals with experiments designed to reconstruct the cellular interactions that bring about T15 dominance. I propose a series of in vivo experiments to analyze the role of antigen, internal images of PC, helper T and B cell subsets in the regulation of T15 expression. Our research addresses important and unresolved issues in immunoregulation: idiotype vs non-idiotype-specific effects, the development of the lymphocyte network and the interdependence of T and B cell repertoires.