TRANSPLANTATION ANTIGENS--EXPRESSION AND FUNCTION

我们计划研究这个专业所编码的第一类基因的生物学。 近交系Balb/c小鼠的组织相容性复合体。 我们之前已经克隆并定位了36个I类基因 两类-三种经典移植基因编码 细胞毒T细胞和33个类I类细胞的限制因子 功能未知的基因。我们计划三个主要项目 实验的类别。(一)表示异国 非免疫组织中的移植抗原(同种异体抗原) (胰腺和肝脏)和选定的免疫相关细胞(B细胞， T细胞和巨噬细胞样细胞)。这些研究应该提供 免疫耐受与H-2机制的研究进展 限制。(2)大量分泌和提纯可溶性 移植抗原以使这些蛋白质结晶并 通过X射线分析确定了它们的三维结构。 我们将在这方面研究一系列KB突变分子 最终，一种移植抗原与 多肽片段。这些研究应该为我们提供对 H-2分子与T细胞受体的分子相互作用 和抗原。(3)我们有针对大多数人的DNA探针 I类基因。我们计划研究36个I类基因的表达 选定的免疫组织和细胞中的基因 非免疫性组织和在分化T和B细胞和 在激活的T细胞中。这些研究应该会提供有用的谱系。 和免疫相关细胞系的分化标志物，并可能 提供对类I类分子的功能的见解。

We plan to study the biology of class I genes encoded by the major histocompatibility complex (MHC) of the inbred Balb/c mouse. We have previously cloned and mapped 36 class I genes falling into two categories-three classical transplantation genes which encode the restriction elements for cytotoxic T cells and 33 class I-like genes whose functions are unknown. We plan three major categories of experiments. (1) To express a foreign transplantation antigen (alloantigens) in nonimmunological tissues (pancreas and liver) and in selected iummune-related cells (B cell, T cell and macrophage-like cells). These studies should provide insights into mechanisms of immunological tolerance and H-2 restriction. (2) To secrete and purify large quantities of soluble transplantation antigens in order to crystallize these proteins and determine their three-dimensional structures by X-ray analysis. We will examine in this regard a series of Kb mutant molecules and eventually a transplantation antigen interacting with a peptide fragment. These studies should provide insights into the molecular interactions between H-2 molecules, T-cell receptors and antigen. (3) We have DNA probes specific for most of the class I genes. We plan to study the expression of the 36 class I genes in immunological tissues and cells, in selected nonimmunological tissues and in differentiating T and B cells and in activated T cells. These studies should provide useful lineage and differentiation markers for immune-related cell lines and may provide insights into the function of the class I-like molecules.