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Transplantation Antigens: Expression and Function

移植抗原：表达和功能

基本信息

批准号：
7214845
负责人：
IWONA T STROYNOWSKI
金额：
$ 33.28万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-06-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7214845
关键词：
Address Adhesions Affect Alternative Splicing Antigens Binding Biological Assay CD94 Antigen CTL assay Cell Line Cell surface Cells Cellular Immunity Class Classification Condition Dependency Family Family member Genes Heat shock proteins Heat-Shock Response Histocompatibility Antigens Histocompatibility Antigens Class I Human Hypoxia Immune response Immune system Immunity Immunization Immunology In Vitro Infection Laboratories Ligands Link Lymphokine-Activated Killer Cells MHC Ib Qa-2 antigen Mammalian Cell Medical Modeling Multiple Myeloma Mus Natural Killer Cells Oxidative Stress Pathway interactions Pattern Peptides Play Population Process Property Protein Family Protein Isoforms Proteins RNA RNA Splicing RNase protection assay Reagent Recombinants Role Science Screening procedure Source Specificity Staining method Stains Standards of Weights and Measures Stress Structure Surface Surveys System T-Cell Receptor T-Lymphocyte Technology Testing Tissues Transcript cell transformation cytotoxic cytotoxicity melanoma novel pathogen prototype receptor research study response stress protein tumor

项目摘要

DESCRIPTION (provided by the applicant): Interplay between innate and adaptive immune response is currently emerging as a novel topic in immunology with a potential impact in medical sciences. Among many different molecules participating in these processes is the family of antigen-presenting class IMHC, which orchestrate NK cell and T cell responses and regulate their interactions. The focus of this proposal is the nonclassical (class Ib) MHC, Qa-2, similar to class la and to human class Ib, HLA-G. Multiple genes and multiple alternatively spliced transcripts with potentially different capabilities or regulating immunity encode Qa-2. Our laboratory played a major role in defining the peptide-binding properties, the crystal structure and the role in NK cell inhibition by GPl-linked, canonical Qa-2. We now plan to use our past expertise and reagents to (1) identify novel isoforms of Qa-2 and determine their expression patterns;(2) evaluate their interactions with components of the innate immune system (NK cells); and (3) study their function in inducing protective adaptive immune response during tumor rejection. The specific hypotheses to be addressed include the notion that peptide-loaded Qa-2 engage NK and T cell receptors and the speculation that they play a specialized role in elimination of stressed/malignantly-transformed cells.

描述（由申请人提供）：先天性和适应性免疫应答之间的相互作用目前正在成为免疫学中的一个新课题，对医学科学具有潜在影响。在参与这些过程的许多不同分子中，有抗原呈递类IMHC家族，其协调NK细胞和T细胞应答并调节它们的相互作用。该建议的重点是非经典（Ib类）MHC，Qa-2，类似于Ia类和人类Ib类，HLA-G。具有潜在不同能力或调节免疫的多个基因和多个可变剪接转录物编码Qa-2。我们的实验室在定义肽结合特性、晶体结构和通过GPl连接的典型Qa-2在NK细胞抑制中的作用方面发挥了重要作用。我们现在计划利用我们过去的专业知识和试剂来（1）鉴定Qa-2的新亚型并确定其表达模式;（2）评估它们与先天免疫系统（NK细胞）组分的相互作用;（3）研究它们在肿瘤排斥反应期间诱导保护性适应性免疫应答的功能。要解决的具体假设包括肽负载的Qa-2参与NK和T细胞受体的概念，以及推测它们在消除应激/恶性转化细胞中发挥专门作用。

项目成果

期刊论文数量（29）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Analysis of MHC class I gene expression in adult bone marrow and fetal liver of the BALB/c mouse.

BALB/c小鼠成年骨髓和胎肝中MHC I类基因表达分析。

DOI：
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
0
作者：
Cheroutre,H;Kronenberg,M;Brorson,K;Hunt3rd,SW;Eghtesady,P;Hood,L;Nickerson,DA
通讯作者：
Nickerson,DA

Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer.

DNA 介导的基因转移后小鼠 L 细胞中 I-Ak II 类基因的表达。

DOI：
10.1038/305440a0
发表时间：
1983
期刊：
Nature
影响因子：
64.8
作者：
Malissen,B;Steinmetz,M;McMillan,M;Pierres,M;Hood,L
通讯作者：
Hood,L

Expression of hybrid class I genes of the major histocompatibility complex in mouse L cells.

小鼠 L 细胞中主要组织相容性复合物的杂合 I 类基因的表达。

DOI：
10.1073/pnas.82.18.6245
发表时间：
1985
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
Straus,DS;Stroynowski,I;Schiffer,SG;Hood,L
通讯作者：
Hood,L

Expression and T cell recognition of hybrid antigens with amino-terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens.

DOI：
10.1084/jem.161.5.935
发表时间：
1985-05-01
期刊：
The Journal of experimental medicine
影响因子：
0
作者：
Stroynowski I;Forman J;Goodenow RS;Schiffer SG;McMillan M;Sharrow SO;Sachs DH;Hood L
通讯作者：
Hood L

H-2Kb antigen expression has no effect on natural killer susceptibility and tumorigenicity of a murine hepatoma.

H-2Kb 抗原表达对小鼠肝癌的自然杀伤敏感性和致瘤性没有影响。