PHYSIOLOGY OF PRE-OVIPOSITION BEHAVIOR

蚊子的行为随着生理状态的变化而变化 关于昆虫的。尽管寻找宿主的行为一般不会 一旦摄入了血餐，就会更长时间地表达出来 卵子发生已经开始，雌性确实变得更受吸引 此时产卵部位的刺激，并展示我们所拥有的 被称为“产卵前期”的寻找产卵地点的行为 鸡蛋。我们的实验室最近证明了 从寻找寄主到产卵前的行为转换可能是 人血淋巴输注非妊娠蚊子的诱导 怀孕的捐赠者。根据我们的初步实验，我们 假设为了显示产卵前，蚊子必须 首先感觉到产卵地点的刺激，必须有成熟的卵巢， 并且必须交配。建议对这一问题进行审查 这三个因素通过哪些机制与 蚊子的中枢神经系统。该计划的时间表 体液产卵前期因子的存在及其稳定性 将首先确定。利用显微外科技术， 交配是如何影响这种行为的，以及交配前 产卵将在确定产卵后终止。 因为在野外饲养的蚊子通常是边缘 滋养的，产卵前行为可能会 将对营养状态不佳的患者进行评估。

Mosquito behavior changes with the changing physiological state of the insect. Although host-seeking behavior is generally no longer expressed once a blood meal has been ingested and oogenesis has commenced, females do become more attracted to oviposition site stimuli at this time, and display what we have termed "pre-oviposition" behavior in search of a place to lay their eggs. Our laboratory has recently demonstrated that the behavioral switch from host-seeking to pre-oviposition can be induced in non-gravid mosquitoes by transfusing hemolymph from gravid donors. Based on our preliminary experiments, we postulate that in order to display pre-oviposition, a mosquito must first perceive oviposition sited stimuli, must have mature ovaries, and must be mated. An examination is proposed of the mechanisms by which these three factors communicate with the central nervous system of the mosquito. The time course for the presence of the humoral pre-oviposition factor and its stability will first be determined. Using techniques of microsurgery, the way in which mating influences this behavior and how pre- oviposition is terminated after oviposition will be determined. Because mosquitoes reared in the field are often marginally nourished, the possibility that pre-oviposition behavior can be modified by suboptimal nutritional state will be evaluated.