ACTIVATION OF THE HUMAN NEURTOPHIL

这些研究旨在研究人的中性粒细胞通过 吞噬和非吞噬刺激，特别是 膜脂作为刺激反应的传导或调节器的作用 耦合。 钙在中性粒细胞的激活过程中扮演着“第二信使”的角色。 细胞内钙的升高是激活后的第一个可测事件 受到各种刺激的影响。CA主要从细胞内动员 商店。尤其是磷脂酰肌醇的分解 磷脂酰肌醇4，5-二磷酸与二酰基的生成 甘油和1，4，5-三磷酸肌醇与该病有关 伴随着中性粒细胞激活的运动。这些脂类的作用和 它们在中性粒细胞激活过程中的衍生物将得到严格的评估。 肿瘤促进剂PMA可以绕过这种钙需求，可能是通过 激活蛋白激酶C；该蛋白激酶C由双酰基激活 甘油，用于改变钙的需要作为各种情况下 需要酶反应。蛋白激酶在细胞周期调控中的作用 调节中性粒细胞的反应将被研究。进一步 钙运动与磷脂相互关系的研究 重塑和蛋白激酶活性应该产生关键的信息 中性粒细胞反应的激活和调节。自.以来 中性粒细胞的适当功能在宿主防御中很重要，因为 以及在炎症性疾病中，对 中性粒细胞的激活顺序具有明显的临床意义。

These studies are designed to study the activation of human neutrophils by phagocytic and non-phagocytic stimuli, with particular reference to the role of membrane lipids as transducers or modulators of stimulus-response coupling. Calcium acts as a "second messenger" in the activation of the neutrophil. A rise in cytosolic Ca is the first measurable event following activation by a variety of stimuli. Ca is mobilized principally from intracellular stores. A role for the breakdown of phosphatidyl inositols, in particular phosphatidyl inositol 4,5 bisphosphate with the generation of diacyl glycerol and inositol 1,4,5 trisphosphate has been implicated in the Ca movements accompanying neutrophil activation. The role of these lipids and their derivatives during neutrophil activation will be critically evaluated. The tumor promoter PMA can bypass this Ca requirement, possibly by activating a protein kinase C; this protein kinase C is activated by diacyl glycerol and serves to modify the Ca requirements as a variety of Ca requiring enzyme reactions. The role of protein kinases in mediating and modulating the neutrophil responses will be investigated. Further investigation into the inter-relation of Ca movements, phospholipid remodeling and protein kinase activity should yield crucial information on the activation and modulation of neutrophil responses. Since the appropriate functioning of the neutrophil is important in host defenses, as well as in inflammatory disorders, a more fundamental understanding of the activation sequence of the neutrophil is of obvious clinical importance.