AUTOIMMUNITY TO NUCLEAR ANTIGENS

本研究项目涉及血清中自身抗体的研究 结缔组织疾病患者的情况。 这些自身抗体产生于 在系统性红斑狼疮患者中自发性，混合性结缔组织 组织疾病、干燥综合征和硬皮病。 许多抗体 与染色体复合的非组蛋白特异性反应。 之一 目的是表征每种核抗原-抗体系统。 迄今为止获得的信息表明，不同非组蛋白的抗体 蛋白质存在于不同的疾病状况中。 换句话说， 如系统性红斑狼疮或硬皮病， 核非组蛋白抗体的特征谱。 因此，在本发明中， 这些抗体特异性的表征可用作标记物 鉴别诊断。 天然存在的自身抗体也被用作试剂， 分离核非组蛋白蛋白。 我们已经描述了一个着丝粒 （动粒）抗原与使用这些试剂抗体之一。 工作 目前的进展是关于分离和定性的其他 与自身免疫抗体反应的非组蛋白染色体蛋白。

This research project is concerned with the study of autoantibodies in the sera of patients with connective tissue diseases. These autoantibodies arise spontaneously in patients with systemic lupus erythematosus, mixed connective tissue disease, Sjogren's syndrome and scleroderma. Many of these antibodies react specifically with non-histone proteins complexed to chromosomes. One of the objectives is to characterize each nuclear antigen-antibody system. Information obtained to date has shown that antibodies to different non-histone proteins are present in different disease conditions. In other words, diseases such as systemic lupus erythematosus or scleroderma have their own characteristic profiles of antibodies to nuclear non-histone proteins. Thus, characterization of the specificities of these antibodies can be used as markers in differential diagnosis. The naturally occurring autoantibodies have also been used as reagents to isolate nuclear non-histone proteins. We have characterized a centromere (kinetochore) antigen with the use of one of these reagent antibodies. Work in progress is currently concerned with the isolation and characterization of other non-histone chromosomal proteins which react with autoimmune antibodies.