AUTOANTIBODIES IN WOMEN WITH SILICONE BREAST IMPLANTS

某些“自身免疫性”疾病是在经历了 使用胶囊硅胶植入物的隆胸手术。 然而，还没有确定这是否是因果关系。 关系或自身免疫性疾病是否在正常人群中发现 预期发病率和流行率与一般人群一样。我们自己的 初步研究表明，uP中存在抗核抗体。 到90%接受硅胶隆胸的女性定义了自身免疫 疾病和一些自身抗体是那些已经 在特发性硬皮病和狼疮中被发现，而其他许多 仍未确认身份。 这项提案的目标是全面描述 患者血清中自身抗体的特征及其特异性 用硅胶隆胸。这将通过以下技术来完成 包括免疫荧光显微镜、Western blotting和 生物合成标记细胞的免疫沉淀抗原性 可以检测到蛋白质和/或与其相关的RNA。它已经做到了 已经证明，有一些新的抗原与来自 硅胶乳房植入物的患者。一些识别的血清 将选择未知抗原作为克隆试剂来探测cdna 表达式库。核酸和蛋白质序列的分析 分离的抗原将被用来确定是否具有 这些抗原中存在结构和功能基序，因为 信息可能会为了解它们的性质提供线索。重组抗原 将从cDNA克隆和真实性中产生 重组蛋白与原血清的酶联免疫吸附试验验证 图书馆放映。然后，将有可能通过ELISA和筛查 大量血清以确定这些自身抗体是否 抗原对于硅胶相关的自身免疫性疾病是独一无二的，或者是 也存在于特发性疾病中。 如果自身抗体图谱和细微的特异性与 自发性自身免疫性疾病，证据将有利于佐剂样 硅胶在促进所谓特发性疾病中的作用 自身免疫性疾病。然而，如果资料和细节显示 尽管如已报道的那样，有某些相似之处，但有 同样明显的差异，证据将争辩说硅胶是 引发自身免疫综合征的不同机制 这些疾病的特发性形式。

Certain "autoimmune" diseases are observed in women who have undergone breast augmentation procedures with encapsulated silicone gel implants. It has, however, not been determined whether this is a cause and effect relationship or whether the auto immune diseases are found in normally expected incidence and prevalence as in the general population. Our own initial studies have shown that antinuclear antibodies are present in up to 90% of women with silicone breast implants who have defined auto immune diseases and that some of the autoantibodies are those which have been recognized in idiopathic forms of scleroderma and lupus while many others remain unidentified. The objective of this proposal is to comprehensively characterize the profiles and fine specificities of autoantibodies in the sera of patients with silicone breast implants. This will be done with techniques which include immunofluorescence microscopy, Western blotting and immunoprecipitation of biosynthetically labelled cells by which antigenic proteins and/or their associated RNAs can be detected. It has already been shown that there are some new antigens reacting with antibodies from patients with silicone breast implants. A number of sera which recognize unknown antigens will be selected as cloning reagents to probe cDNA expression libraries. Analysis of the nucleic acid and protein sequences of the isolated antigens will be used to determine if characteristic structural and functional motifs are present in these antigens since this information might provide leads to their nature. Recombinant antigens will be generated from the cDNA clones and the authenticity of the recombinant proteins verified in ELISA with the original sera used for library screening. It will then be possible with ELISA and screening of large numbers of sera to ascertain whether autoantibodies to these antigens are unique for silicone-associated auto immune diseases or are also present in the idiopathic diseases. If autoantibody profiles and fine specificities are not different from spontaneous autoimmune diseases, the evidence would favor an adjuvant-like effect of silicone in the promotion of what has been called idiopathic autoimmune disease. However, if the profiles and specificities show that although there are certain similarities as has been reported, there are also distinct differences, the evidence would argue that silicone was provoking autoimmune syndromes by additionally different mechanisms from the idiopathic forms of these diseases.