NUCLEIC ACID AND PROTEIN METABOLISM IN CARTILAGE

本研究建议的目的是继续研究正常的 以及病理性关节和骺软骨， 健康和各种疾病状态下组织的代谢， 尤其是人骨关节炎。 将进行的研究包括 继续研究正常和病理组织中的DNA复制活性 软骨，特别强调流式细胞术的使用;续 定义正常和病理的产物和合成率 软骨利用各种生化和代谢技术 包括无细胞系统;对各种正常 和代谢的病理控制机制（合成代谢和 分解代谢）活动的正常和异常软骨;寻找 细胞内和细胞膜标志物的疾病状态， 病理软骨，主要通过使用流式细胞仪;并继续 尝试分离、鉴定、纯化和测定 降解的最佳条件、底物、辅因子和抑制剂 酶在正常和患病的关节和骨骺软骨。

The purpose of this research proposal is to continue the study of normal and pathological articular and epiphyseal cartilage with special attention to the metabolims of the tissue in health and a variety of disease states, especially human osteoarthritis. The studies to be performed include a continued study of DNA replicative activity in normal and pathological cartilages with special emphasis on the use of flow cytometry; a continued definition of the products and rates of synthesis of normal and pathologic cartilages utilizing a variety of biochemical and metabolic techniques including the cell-free systems; a continued study of a variety of normal and pathological control mechanisms of the metabolic (both anabolic and catabolic) activities of normal and abnormal cartilages; a search for intracellular and cell membrane markers of disease states in normal and pathologic cartilages, chiefly by use of the flow cytometer; and continued attempts at isolation, identification, purification and determination of optimal conditions, substrate, cofactors and inhibitors for degradative enzymes in normal and diseased articular and epiphyseal cartilages.