HUMAN ANTI-H. INFLUENZAE B IDIOTYPES

尽管有抗生素可用，流感嗜血杆菌b 仍然是导致儿童发病和死亡的主要原因。 这项研究建议分析人类的独特型多样性 针对猪瘟病毒主要抗原和毒力因子的抗体 流感嗜血杆菌B，囊状碳水化合物，多聚核糖基核糖醇- 磷酸盐(PRP)。利用杂交瘤方法，小鼠 猪传染性支气管炎交叉反应性独特异表位的特异性单抗 将制备PRP特异性抗体。这些抗独特症 试剂将被用来确定：白痴的程度 在没有血缘关系的人类之间共享，无论是婴儿还是成年人 表达相似的独特型曲目，以及是否反独特型 抗体可以使新生大鼠免受H。 乙型流感病毒感染。此外，还将尝试确定 同源人类抗独特型抗体。这些研究将 提供对可变区域多样性和个体发育的洞察 人源抗PRP抗体库以及检测 使用抗独特型抗体作为保护性H. 乙型流感疫苗。人类独特型的鉴定与抗-抗 独特型对于未来的研究是必要的，这些研究试图确定 抗独特型抗体是否调节B细胞的表达 婴儿和成年人。

Despite the availability of antibiotics, Hemophilus influenzae b remains a leading cause of morbidity and mortality in children. This study proposes to analyze the idiotypic diversity of human antibodies specific for the major antigen and virulence factor of H. influenzae b, the capsular carbohydrate, polyribosyl-ribitol- phosphate (PRP). Using hybridoma methodology, murine monoclonal antibodies specific from cross-reactive idiotopes of PRP-specific antibodies will be prepared. These anti-idiotopic reagents will be used to determine: the extent to which idiotopes are shared between unrelated humans, whether infants and adults express similar idiotypic repertoires, and whether anti-idiotopic antibodies can prime neonatal rats for protection from H. influenzae b infection. Also, attempts will be made to identify isologous human anti-idiotypic antibodies. These studies will provide insight into the variable region diversity and ontogeny of the human anti-PRP antibody repertoire as well as test the feasibility of using anti-idiotypic antibodies as a protective H. influenzae b vaccine. Identification of human idiotypes and anti- idiotypes is necessary for future studies which seek to determine whether anti-idiotypic antibodies regulate B cell expression in the infant and the adult.