THICK FILAMENT MYOSIN AND C-PROTEIN ARRANGEMENT

肌球蛋白及其附属蛋白如C蛋白在肌球蛋白中的排列 脊椎动物的横纹肌粗丝尚不完全清楚。近期 然而，对青蛙粗丝结构的实验已经 证明脊椎动物粗丝的结构可能是 可通过电子显微镜、光学显微镜、 衍射分析和计算机图像重建技术。这个 拟议研究的具体目的是扩大这些研究的范围 并将研究扩展到青蛙粗丝的测定和 鱼类、禽类、家禽等鱼类粗丝结构的比较 和哺乳动物的横纹肌。这些研究的主要目标是 是肌球蛋白的排列和数量的测定 这些细丝中的交叉桥水平以及C蛋白是如何相对排列的 到潜在的肌球蛋白。为实现这些目标而进行的研究将 包括：1)开发隔离原生动物的程序 细丝粗大，结构变化极小，分别来自 物种；2)电子显微镜和光学技术的结合 用衍射法对细丝进行显微分析 3)利用计算机图像分析技术对细丝进行分析 图像在最好的电子显微照片中确定旋转 肌球蛋白头部的对称性和排列，以及肌球蛋白头部的排列 C蛋白。这些研究将提供有关 肌肉的结构，因此将提供更好的基础 了解病理条件如何影响这一功能 组织。

The arrangement of myosin and accessory proteins such as C-Protein in the vertebrate striated muscle thick filament is incompletely known. Recent experiments on the structure of frog thick filaments, however, have demonstrated that the structure of vertebrate thick filaments may be amenable to analysis by a combination of electron microscopy, optical diffraction analysis, and computer image reconstruction techniques. The specific aim of the proposed research is to expand these studies of the frog thick filaments and to extend the studies to the determination and comparison of the structure of thick filaments isolated from fish, avian, and mammalian striated muscle. The major objective of these studies will be the determination of the arrangement and number of myosins per crossbridge level in these filaments and how C-protein is arranged relative to the underlying myosin. The studies to accomplish these aims will include: 1) the development of procedures fro the isolation of native thick filaments with minimal structural alteration from each of the species; 2) the use of combination of electron microscopy and optical diffraction analysis of the micrographs to assess the filament preservation; and 3) the use of computer image analysis of the filament images in the best electron micrographs to determine the rotational symmetry and arrangement of the myosin heads, and the arrangement of C-protein. These studies will provide basic scientific information about the structure of muscle, and will thus provide a better foundation for understanding how pathologic condition may affect the functioning of this tissue.