MONOCLONAL ANTIBODIES TO HUMAN EPIDERMIS

针对表皮和基底膜带抗原的单抗有 已经形成了新的抗体，通过融合来自 用小鼠骨髓瘤细胞系P3/NS1免疫小鼠。免疫的小鼠 与人宫颈癌细胞系(ME-180)形成抗体 到人类锚定的纤维。人类皮肤和ME-180中的抗原将是 完全用它的分子量，等电点， 多肽和溴化氰图谱及氨基酸组成。单元格 皮肤中抗原的来源将通过生物合成研究来确定 以及裸鼠身上的真皮/表皮重组。形成了一种 锚定原纤维和它的抗原将在 创面愈合和表皮细胞附着于胶原蛋白涂层期间 组织培养皿。锚定原纤维抗原Will的基因定位 利用ME-180细胞的体细胞杂交技术进行 将其与聚乙二醇与小鼠表皮细胞融合。 锚定原纤维抗原已与许多人类皮肤样本发生反应。 但对营养不良患者的非水泡皮肤无反应 大疱性表皮松解症，一种锚定纤维缺失的疾病 电子显微镜显示。尝试使用锚定纤维 大疱性表皮松解症治疗后的抗体将由 患者的活组织检查，并通过跟踪患者的皮肤活组织检查 大疱性表皮松解症移植到裸鼠身上。在以下方面成功 这些技术鼓励我们使用这些技术来形成抗体 这将允许解剖异质的表皮种群 基细胞，也是区分基细胞和非基细胞的标志。

Monoclonal antibodies to epidermal and basement membrane zone antigens have been formed and new antibodies will be formed by fusing spleen cells from immunized mice with the mouse myeloma cell line P3/NS1. Mice immunized with a human cervical carcinoma cell line (ME-180) have formed an antibody to human anchoring fibrils. The antigen in human skin and ME-180 will be completely characterized with its molecular weight, isoelectric point, peptide and cyanogen bromide map and amino acid composition. The cell of origin of the antigen in skin will be determined by biosynthetic studies and dermal/epidermal recombinations on the nude mouse. The formation of the anchoring fibril and its antigen will be sequentially followed during wound healing and during epidermal cell attachment to collagen-coated tissue culture dishes. Gene mapping of the anchoring fibril antigen will be performed by somatic cell hybridization techniques using ME-180 cells which will be fused with polyethylene glycol with mouse epidermal cells. The anchoring fibril antigen has reacted with many samples of human skin but is non-reactive with the non-blistered skin of patients with dystrophic epidermolysis bullosa, a disease in which anchoring fibrils are absent as shown by electron microscopy. Attempts to use the anchoring fibril antibody to follow therapy of epidermolysis bullosa will be made both by biopsies of patients and by following the skin biopsies of the epidermolysis bullosa skin transplanted to the nude mouse. Success with these techniques encourages us to use these techniques to form antibodies which will allow dissection of the heterogeneous population of epidermal basal cells and also which distinguishes basal from non-basal cells.