WATCHING TARGET CELL OXIDATION AND CYTOLYSIS

跨学科研究计划将直接观察活细胞 当它们氧化和裂解靶细胞时，对免疫系统产生影响。氧化 而靶细胞的细胞溶解以前从未被观察到 光学显微镜。新的生物和物理技术现在使这一点 有可能。此外，效应细胞组织它们的方式 将探索进行这些活动的质膜。三 在这些研究中将使用模型靶标：1)绵羊红细胞，2) 8E5细胞，已感染人类免疫缺陷病毒；3) 肿瘤细胞。 中性粒细胞介导的免疫球蛋白调理红细胞的氧化和细胞溶解 将通过Soret带透射式光学显微镜和曙红Y观察到 分别介绍了标记方法。氧化分子的作用将是 使用慢性肉芽肿性疾病中性粒细胞进行研究。细胞表面 中性粒细胞Fc受体在抗体前后的分布 依赖的细胞细胞毒性将被确定。类似的一系列 实验将在C3双向调光红细胞上进行， 补体受体CR3。这些结果将与细胞溶解进行比较 淋巴细胞的作用机制(S)。 中性粒细胞和巨噬细胞介导的8E5和肿瘤细胞的氧化 将会被研究。有核的靶细胞的胞浆将被标记 使用对氧化条件敏感的荧光化合物。这个 靶细胞过程中效应细胞表面Fc受体的特性 氧化将在抗体依赖的细胞毒性过程中进行研究。 抗体非依赖性细胞毒作用过程中肿瘤靶点的氧化 也将进行研究。

The interdisciplinary research program will directly observe living cells of the immune system as they oxidize and lyse target cells. The oxidation and cytolysis of target cells have never previously been observed by optical microscopy. New biological and physical technologies now make this possible. Furthermore, the manner in which effector cells organize their plasma membranes to carry out these activities will be explored. Three model targets will be employed in these studies: 1) sheep erythrocytes, 2) 8E5 cells, which have been infected by human immunodeficiency virus, and 3) tumor cells. Neutrophil-mediated oxidation and cytolysis of IgG-opsonized erythrocytes will be observed by Soret band transmitted light microscopy and eosin Y labeling methods, respectively. The role of oxidative molecules will be studied using chronic granulomatous disease neutrophils. The cell surface distribution of neutrophil Fc receptors before, during, and after antibody- dependent cellular cytotoxicity will be determined. A similar series of experiments will be conducted on C3bi-opsonized erythrocytes and the complement receptor CR3. These results will be compared to the cytolytic mechanism(s) of lymphocytes. The neutrophil- and macrophage-mediated oxidation of 8E5 and tumor cells will be studied. The cytosol of nucleated target cells will be labeled with fluorescent compounds that are sensitive to oxidative conditions. The properties of effector cell surface Fc receptors during target cell oxidation will be studied during antibody-dependent cellular cytotoxicity. The oxidation of tumor targets during antibody-independent cytotoxicity will also be studied.