FLOW CYTOMETRIC ANALYSIS OF PBMC AFTER HIV-CHEMOTHERAPY

这个项目的长期目标是进一步开发一种新的 免疫荧光与Flow结合的技术 细胞学。这项技术将通过比较来自 流式细胞仪技术与源于定量的 聚合酶链式反应(PCR)和传染性测定。验证 这项新的流式细胞术将提供一种相对 一种廉价、快速的数量检测和定量方法 HIV感染的外周血单个核细胞(PBMC) 血清阳性的人。这项技术将被用于监测疾病 艾滋病病毒感染者的进展和监测抗病毒药物的效果 逆转录病毒化疗对这些人的病毒负荷的影响。 这项拨款建议的具体目标是： 1.通过比较获得的数据来验证流式细胞仪技术 流式细胞仪检测结果与聚合酶链式反应和感染力测定结果进行比较。 2.使用流式细胞仪技术监测正在 接受抗逆转录病毒化疗，如ZDV、DDC和DDI。 3.用流式细胞仪技术鉴定细胞。 感染艾滋病毒的外周血液。 4.用流式细胞仪鉴定外周血中的细胞 感染巨细胞病毒(CMV)的血液。检测和 定量检测感染CMV的PBMC可能有助于阐明CMV的作用 这种机会性感染在艾滋病的发病机制中发挥了作用。 这些研究的成功完成将验证一种技术 将使对感染艾滋病毒的PBMC数量的监测迅速和 相对便宜。

The long term goal of this project is to develop further a novel technique involving immunofluorescence in conjunction with flow cytometry. This technique will be validated by comparing data from the flow cytometric technique with that derived from the quantitative polymerase chain reaction (PCR) and infectivity assays. Validation of this novel flow cytometric technique will provide a relatively inexpensive and rapid method for detecting and quantitating the number of HIV-infected peripheral blood mononuclear cells (PBMC) in HIV- seropositive individuals. This technique will be used to monitor disease progression in HIV-infected individuals and monitor the effects of anti- retroviral chemotherapies on virus burden in these individuals. The specific aims of this grant proposal are: 1. Validate the flow cytometric technique by comparing data obtained from flow cytometry with that derived from PCR and infectivity assays. 2. Use the flow cytometric technique to monitor patients who are receiving anti-retroviral chemotherapy such as ZDV, ddc and ddi. 3. To use the flow cytometric technique to identify the cells in the peripheral blood that are infected with HIV. 4. To use the flow cytometric to identify the cells in the peripheral blood that are infected with cytomegalovirus (CMV). Detection and quantitation of the PBMC infected with CMV may help elucidate the role that this opportunistic infection plays in the pathogenesis of AIDS. Successful completion of these studies will validate a technique that will make monitoring of the number of HIV-infected PBMC rapid and relatively inexpensive.