THE GLUCOREGULATORY PEPTIDE HORMONES

在接下来的一年里，我们希望能够描述人类D细胞的功能。 经过一年的努力，我们还没有开发出一种实用的放射免疫测定法， 生长抑素在未提取的人血浆中，就像我们以前在狗身上做的那样。 我们 已经定义了这种分析的某些技术障碍， 内源性生长抑素的快速降解， 对生长抑素示踪剂的破坏，构成主要障碍。 我们计划 测试四种提取或分离工艺的效果， 避免了全血浆分析的问题。 这些包括凝胶过滤， 丙酮提取、免疫亲和层析和离子交换 层析 迄今为止，观察到的最佳回收率为 免疫亲和层析方法。 我们现在的努力是为了 这些技术对于生理学所需大体积测定工作是实用的， 人类的病理生理学研究。如果完成了这项工作，我们计划 研究生长抑素对正常人进餐和其他动作的反应， 受试者和肥胖受试者以及各种形式的糖尿病中， 胃肠道疾病和垂体疾病。 我们对这个问题的兴趣 促生长素抑制素作为营养调节剂， 肠腔向内部环境的运动可能在 广泛的人类疾病 与Raskin和Pietri博士合作，对 I型糖尿病患者在肌毛细血管基底的代谢接近正常化 膜厚度、肾功能、运动神经传导速度、HDL-2和 低密度脂蛋白水平，视网膜荧光素血管造影将继续进入第二次 年

During the coming year we hope to characterize D-cell function in man. After one year of effort, we have yet to develop a practical radioimmunoassay for somatostatin in unextracted human plasma, as we had done previously in dogs. We have defined certain of the technical obstacles to such an assay and it appears that rapid degradation of endogenous somatostatin, coupled with proteolytic damage to the somatostatin tracer, constitute the main obstacles. We plan to test the efficacy of four extraction or isolation technics by which to circumvent the problems of whole plasma assay. These include gel filtration, acetone extraction, immunoaffinity chromatography and ion exchange chromatography. The best recoveries have thus far been observed with immunoaffinity chromatography methods. Our efforts now are designed to make such technics practical for large volume assay work required for physiologic and pathophysiologic studies in man. If and when this is accomplished, we plan to study the somatostatin response to meals and to other maneuvers in normal subjects and in obese subjects as well as in the various forms of diabetes, in gastrointestinal disorders and pituitary disease. Our interest in this problem is prompted by the possibility that somastatin, as the regulator of nutrient movement of the gut lumen to the internal environment, may be important in a broad spectrum of human disorders. In collaboration with Drs. Raskin and Pietri a clinical study of the efficacy of metabolic near-normalization of Type I diabetics upon muscle capillary basement membrane thickness, renal function, motor nerve conduction velocity, HDL-2 and LDL levels, and retinal fluorescin angiography will continue into its second year.