DIETARY TRYPTOPHAN IN CONTROLLING PROTEIN SYNTHESIS

这项调查的目的是探索一种单一的必需氨基酸如何 酸，特别是L色氨酸，会影响肝脏的蛋白质代谢。 在我们实验室早先的报告中，我们描述了L-色氨酸 快速刺激肝脏多聚核糖体聚集和蛋白 综合。这种影响似乎与两者的改变有关。 转录和转录后控制。我们目前的建议 计划在细胞和亚细胞水平上探索L-色氨酸 可能直接作用于核(核膜)以刺激 核质转位增强。我们计划在北京学习。 详细说明肝细胞胞浆和核膜的蛋白质是如何 色氨酸处理的大鼠参与了核浆的增加 信使核糖核酸易位。此外，我们计划研究色氨酸在人体内的作用。 肝毒作用对大鼠肝脏蛋白质合成的影响 探员们。这样的信息可以提供基本的洞察，了解一个重要的 饮食成分，如L-色氨酸，可能会计划一种 在哺乳动物肝脏蛋白质代谢中的作用。

This investigation is interested in exploring how a single essential amino acid, specifically L-tryptophan, affects protein metabolism in the liver. In earlier reports from our laboratory we have described that L-tryptophan rapidly stimulates hepatic polyribosomal aggregation and protein synthesis. This effect appears to be related to alterations in both transcriptional and postranscriptional controls. Our present proposal plans to explore at the cellular and subcellular levels how L-tryptophan may act, possibly directly on the nucleus (nuclear membrane), to stimulate enhanced nucleocytoplasmic translocation of mRNA. We plan to study in detail how proteins of the cytosol and nuclear membranes of the livers of tryptophan-treated rats are involved in the increased nucleocytoplasmic translocation of mRNA. Also, we plan to investigate how tryptophan acts in improving hepatic protein synthesis in rats treated with hepatotoxic agents. Such information may offer basic insight as to how an important dietary component, as exemplified by L-tryptophan, may plan a regulatory role in mammalian liver protein metabolism.