CONTROL OF VIRAL RNA SYNTHESIS IN HERPESVIRUS INFECTION

我们将继续进行HSV-1mRNA图谱的研究，以完成我们的 大小、位置、体外翻译产品和时间的描述 单纯疱疹病毒基因组中主要病毒mRNAs图谱的类别。以上数据， 以及其他地方描述的核苷酸序列分析，将引导我们 定义典型的早期(Beta)和晚期(Beta Lambda和Lambda) “推动者。”我们将通过检查以下产品继续我们的分析 模型HSV-1基因转录。我们将使用来自 感染和未感染的细胞。我们还将介绍典型的HSV-1 使用最近描述的几种方法之一将基因导入细胞 其他。一旦这些措施被引入，我们将能够确定其效果 病毒重叠感染对HSV-1类基因表达的影响。我们 将操纵启动子区域的序列以确认它们的作用 在控制病毒基因表达方面。我们将调查 上述确定的HSV-1特异性基因的生物学功能。我们摆出两个姿势 攻击途径：(A)与G.Cohen教授和R. 宾夕法尼亚大学的艾森伯格利用他们收集的 抗病毒结构蛋白多特异性和单特异性抗血清 研究特定的体外翻译产品；(B)与 加州大学洛杉矶分校的J·史蒂文斯教授在他的 标记性抢救研究试图识别涉及的特定病毒基因 在单纯疱疹病毒病理的不同方面。

We will continue HSV-1 mRNA mapping studies in order to complete our description of size, location, in vitro translation products, and temporal class of the major viral mRNAs mapping in the HSV genome. The above data, as well as nucleotide sequence analysis described elsewhere, will lead us to define typical early (Beta) and late (Beta Lambda and Lambda) "promoters." We will continue our analysis by examining the products of transcription of moDel HSV-1 genes. We will use in vitro systems from infected and uninfected cells. We also will introduce prototypical HSV-1 genes into cells using one of several methods recently described by others. Once these are introduced, we will be able to determine the effect of viral superinfection on the expression of classes of HSV-1 genes. We will manipulate the sequences of the promoter regions to confirm their role in the control of viral gene expression. We will investigate the biological function of specific HSV-1 genes identified above. We pose two avenues of attack: (a) collaboration with Professors G. Cohen and R. Eisenberg of the University of Pennsylvania to use their collection of poly- and monospecific antisera against viral structural proteins to investigate specific in vitro translation products; (b) collaboration with Professor J. Stevens of the University of California, Los Angeles, in his marker rescue studies to attempt to identify specific viral genes involved in different aspect of HSV pathology.