DEVELOPMENT OF CLINICAL APPLICATIONS OF GC/MS

本研究的目的是开发和应用组合技术。 气相色谱/质谱仪用于碎裂的研究， 生物化学和生物化学核苷的代谢、分布和排泄 临床兴趣。要成功实现这些目标，需要 稳定的同位素标记核苷的合成 对于这些分子的碎裂，技术的发展 络合物中特定分子的分离和鉴定 从生物基质中分离出的化合物的混合物及其定量 具有临床重要性的核苷及其代谢产物 从生物样本中分离出的抗癌药物。这些原则的应用 涉及主题的研究技术是这项工作的最终目标 并正在努力建立必要的协作 协议。这项工作的结果将在 设计抗肿瘤药物给药的给药方案 设计具有增强抗肿瘤活性的核苷类似物。 这些技术也可能在癌症和癌症的诊断中具有重要意义 在跟踪癌症治疗的疗效方面，通过监测 癌症患者尿中修饰核苷排泄量的研究 在治疗之后。

The aim of this research is to develop and apply the techniques of combined gas chromatography/mass spectrometry to the study of the fragmentation, metabolism, distribution and excretion of nucleosides of biochemical and clinical interest. The successful attainment of these objectives requires the synthesis of stable isotopically labeled nucleosides, an understanding of the fragmentation of these molecules, the development of techniques for the separation and identification of specific molecules in a complex mixture of compounds isolated from a biological matrix and the quantitation of nucleosides, and their metabolities, of clinical importance as anticancer agents isolated from biological samples. Application of these techniques to studies involving subjects is the ultimate goal of this work and efforts are being made to establish the necessary collaborative agreements. The results of this work would be of significant value in designing dosage schedules for the administration of antitumor agents and in the design of nucleoside analogs with increased antitumor activity. These techniques may also be of significance in the diagnosis of cancer and in following the efficacy of the treatment of cancer by monitoring the urinary excretion of modified nucleosides by cancer patients both before and after therapy.