REGULATION OF CELLS BY MATRIX AND HORMONES

我们的实验室主要研究 细胞外基质，并与基质和激素之间的协同作用， 调节细胞生理学 多年来，我们一直 开发必要的技术，研究矩阵的作用， 激素在调节分化哺乳动物基因表达中的作用 上皮样细胞，肝细胞，以及它们的肿瘤对应物， 肝癌 这些技术使我们能够培养正常的肝细胞 或肝细胞瘤，使它们要么以最大速度生长， 肝脏特异性功能的水平或维持为非生长细胞 具有更高水平的肝脏特异性功能。 述技术包括 使用无血清、经尿道确定的培养基和浸提物基质 含有称为生物基质的细胞外基质。 目前我们 用化学方法解剖生物基质，以确定哪些分子 负责其生物活性，并利用信息形成 由确定的和纯化的基质成分组成的基质。 初始 研究已经单独使用已知的基质组分， 组合。 在用它们的组分优化这些培养物之后， 我们将把生物基质提取物作为 纯化具有生物活性的其它基质组分。 的 项目涉及生物和化学技术，所以我们有 融合了L博士的专业知识罗森伯格，谁拥有净化经验， 和蛋白聚糖的特性，E。J.米勒，他有经验， 胶原蛋白的纯化和表征，以及L. Reid手机 在培养分化的哺乳动物方面有专长的生物学家 上皮细胞 这个合资企业应该最大限度地提高 成功开发用于生长的明确基质，和/或 上皮细胞的分化。 （一）

Our laboratories are concerned with the chemistry and biology of extracellular matrix and with the synergies between matrix and hormones in regulating cellular physiology. For a number of years we have been developing the technologies necessary to study the roles of matrix and hormones in regulating gene expression in a differentiated mammalian epithelioid cell, the hepatocytes, and in their neoplastic counterparts, the hepatomas. These technologies permit us to culture normal hepatocytes or hepatomas so that they either grow at maximal rates and with lower levels of liver-specific functions or are maintained as non-growing cells with higher levels of liver-specific functions. The technologies include the use of serum-free, hormonally defined media and substrata of extracts containing extracellular matrix referred to as biomatrix. At present, we are chemically dissecting the biomatrix to ascertain which molecules are responsible for its biological activity and using the information to form substrata composed of defined and purified matrix components. The initial studies have utilized known matrix components alone and in various combinations. After optimization of these cultures with their components, we will turn to the biomatrix extracts as a source material for the purification of other matrix components with biological activity. The project involves the technologies of both biology and chemistry, so we have fused the expertise of Dr. L. Rosenberg, who has experience in purifying and characterizing proteoglycans, Dr. E. J. Miller, who has experience in purification and characterization of collagens, and Dr. L. Reid, a cell biologist who has expertise in culturing differentiated mammalian epithelial cells. This joint venture should maximize the chances of success in developing defined matrix substrata for growth and/or differentiation of epithelial cells. (A)