TRANSFORMATION BY ABELSON MURINE LEUKEMIA VIRUS

这项提议将研究涉及到的分子机制 Abelson转化的小鼠淋巴细胞的异常生长调节 鼠白血病病毒(A-MuLV)。详细评估了遗传和 A-MuLV转化基因的酶学性质及其正常 将进行细胞同源分析。改良血清学试剂盒的制备 和酶底物试剂将用合成的多肽 技术野生型和突变型，包括部分或完全 转化有缺陷的菌株、非致死变种和温度 敏感株将用于研究病毒之间的相互作用。 磷酸酪氨酸激酶与宿主细胞环境的关系。世俗的 正常B淋巴样细胞的分化过程和表型进展 在感染A-MuLV后，将遵循向转变状态的方向 使用新开发的细胞培养技术(Whitlock和Witte，1982a)。 这些研究将更好地确定A-MuLV靶细胞的性质和 充分表达转化状态所需的细胞因子 淋巴样细胞。

This proposal will investigate the molecular mechanisms involved in the abnormal growth regulation of murine lymphocytes transformed by the Abelson murine leukemia virus (A-MuLV). A detailed evaluation of the genetic and enzymatic properties of the A-MuLV transforming gene and its normal cellular homolog will be carried out. Preparation of improved serological and kinase substrate reagents will be done using synthetic peptide technology. Both wild type and mutants including partial or complete transformation defective strains, non-lethal variants, and temperature sensitive strains will be used to study the interaction of the viral phosphotyrosine kinase with the host cell environment. The temporal process and phenotypic progression of normal B lymphoid cells post-infection with A-MuLV towards the transformed state will be followed using newly developed cell culture techniques (Whitlock and Witte, 1982a). These studies will better define the nature of the A-MuLV target cell and cellular factors required for full expression of the transformed state in lymphoid cells.