CUKPGP: Streptococcus suis in the pig meat supply chain: from genomics to control strategies.

猪链球菌是猪的一种重要的人畜共患病病原体，对猪的生产和人类健康具有全球性的影响。为了保护人类健康和减少生猪生产对抗菌剂的依赖，需要制定SS控制策略。该项目旨在确定预防猪和人SS相关疾病的控制点。在英国和中国的农场和屠宰场(密集和传统系统)进行的纵向研究，以及分离株的全基因组测序，将通过揭示供应链上的菌株多样性、适应性和抗菌素耐药性，为疾病预防的新控制点提供指导。序列将与从猪肉产品中获得的序列和从人类分离株中获得的序列进行比较。将调查在消费者即食猪肉中携带SS的风险因素。我们将使用转座子定向插入位点测序(TRADIS)来确定SS的环境控制途径，以探索支持SS在农场、屠宰场和肉制品环境中生存的基因。所有合作伙伴(剑桥大学、华中农业大学(HAU)和惠康信托桑格研究所(WTSI))在SS研究方面都有良好的记录。CU和Hau拥有中国合作奖，三人都在现有的BBSRC更长期和更大规模的资金支持的财团中合作，这是一种针对猪细菌性呼吸道疾病的单一平台诊断和多价疫苗方法。

Streptococcus suis (SS) is an important zoonotic pathogen of pigs with global relevance to pig productivity and human health. Strategies for SS control are needed to protect human health andto reduce dependence of pig production on antimicrobials. This project aims to identify control points for the prevention of SS related disease in pigs and humans. Longitudinal studies in UK and Chinese farms and abattoirs (intensive and traditional systems), with whole genome sequencing of isolates, will provide guidance to new control points for disease prevention by shedding light on strain diversity, fitness, and antimicrobial resistance along the supply chain. Sequences will be compared to those obtained from pig meat products and those available from human isolates. Risk factors for carriage of SS on consumer-ready pig-meat will be investigated. We will identify avenues for environmental control of SS using transposon directed insertion site sequencing (TraDIS) to explore the genes that underpin environmental survival of SS in the farm, abattoir and meat product context.All partners (Cambridge University, Huazhong Agricultural University (HAU), and Wellcome Trust Sanger Institute (WTSI)) have strong track records in SS research. CU and HAU hold a China Partnering Award, and all 3 work together within the existing BBSRC Longer and Larger funded consortium, 'A single platform diagnostic and multivalent vaccine approach to bacterial respiratory diseases of pigs'.