Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014.
Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014.
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DOI：
10.1016/j.vetmic.2017.06.002
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发表时间：
2017-08
影响因子：
3.3
通讯作者：
BRADP1T Consortium
中科院分区：
文献类型：
--
作者：
Hernandez-Garcia J;Wang J;Restif O;Holmes MA;Mather AE;Weinert LA;Wileman TM;Thomson JR;Langford PR;Wren BW;Rycroft A;Maskell DJ;Tucker AW;BRADP1T Consortium
疾病和非疾病相关分离株具有不同的耐药性。临床分离株的抗生素耐药性随着时间的推移而增加。对多种抗菌药物具有耐药性的分离株的患病率正在增加。不同方法的组合增强了从数据中获取的信息。猪链球菌是一种全球性的猪人畜共患病原体，其抗菌药物耐药性大多仅在患病动物中进行研究，调查并未评估随时间的变化。我们比较了英国临床病例 (CC) 和非临床病例 (NCC) 猪中猪链球菌分离株的耐药模式，这些猪是在 2009-2011 年和 2013-2014 年两个独立时期收集的。对按采样周期和疾病关联分类的 405 株猪链球菌分离株测定了 17 种抗菌药物（九类）的最低抑菌浓度 (MIC)，以评估耐药性随时间的变化以及与疾病的关联。首先，使用已发表的临床断点将分离株鉴定为耐药或敏感。其次，流行病学临界值 (ECOFF) 源自 MIC 值，并将分离株分类为低于 ECOFF 的野生型 (WT) 和高于 ECOFF 的非野生型 (NWT)。最后，分析分离子集的 MIC 分布变化。在两个研究期间，NCC 分离株比 CC 分离株对头孢菌素、青霉素、截短侧耳素、强效磺胺类药物和四环素类抗生素的耐药性更强。相对于 2009-2011 年，2013-2014 年 CC 分离株对氨基糖苷类、头孢菌素类、氟喹诺酮类、截短侧耳素、强效磺胺类和四环素类抗菌药物的耐药水平有所上升。在两个时间段内，NCC 中被归类为 NWT 的五类或更多类抗菌药物的分离株的患病率均高于 CC 分离株，并且随着时间的推移而增加。这项研究使用标准化方法来确定从英格兰猪中分离出的猪链球菌耐药性表型的显着变化，不仅随着时间的推移，而且在已知临床病例或无病猪的分离株之间也发生了显着变化。
Disease and non-disease associated isolates had different resistance profiles. Antimicrobial resistance increased over time among clinical isolates. Prevalence of isolates with resistance to multiple antimicrobials is increasing. Combination of different approaches enhances the information obtained from the data. Antimicrobial resistance in Streptococcus suis, a global zoonotic pathogen of pigs, has been mostly studied only in diseased animals using surveys that have not evaluated changes over time. We compared patterns of resistance between S. suis isolates from clinical cases of disease (CC) and non-clinical case (NCC) pigs in England, collected over two discrete periods, 2009–2011 and 2013–2014. Minimum inhibitory concentrations (MIC) of 17 antimicrobials (nine classes) were determined on 405 S. suis isolates categorised by sampling period and disease association to assess changes in resistance over time and association with disease. First, isolates were characterized as resistant or susceptible using published clinical breakpoints. Second, epidemiological cut-offs (ECOFF) were derived from MIC values, and isolates classified as wild type (WT) below the ECOFF and non-wild type (NWT) above the ECOFF. Finally, isolate subsets were analysed for shifts in MIC distribution. NCC isolates were more resistant than CC isolates to cephalosporins, penams, pleuromutilins, potentiated sulphonamides and tetracyclines in both study periods. Resistance levels among CC isolates increased in 2013–2014 relative to 2009–2011 for antimicrobials including aminoglycosides, cephalosporins, fluoroquinolones, pleuromutilins, potentiated sulphonamides and tetracyclines. The prevalence of isolates categorised as NWT for five or more classes of antimicrobials was greater among NCC than CC isolates for both time periods, and increased with time. This study used standardised methods to identify significant shifts in antimicrobial resistance phenotypes of S. suis isolated from pigs in England, not only over time but also between isolates from known clinical cases or disease-free pigs.
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