INCIDENCE, QUANTITATION OF A TUMOR PROTEIN (ONCOMODULIN)

肿瘤转化改变了几种细胞的基础浓度， 钙结合蛋白、钙调蛋白、S-100和皮肤CaBP。 的 肿瘤发育钙结合蛋白肿瘤调节蛋白也可能出现在 肿瘤，但与其他肿瘤不同的是， 正常啮齿动物或成人组织，并出现在85%的肿瘤， 不同的起源。 这是长期的目标，以适应外观， 癌调节蛋白进入钙转运的一般扰动， 调节蛋白质，必须负责某些部分的 肿瘤表型 我们必须确定， 癌调节蛋白基因与特定癌基因的功能有关，或 将病毒携带的致癌基因插入细胞的特定部位 基因组，或者是肿瘤发展的一般反应。 评价很 感兴趣的是，用来自化学方法的DNA转染正常细胞， 转化细胞激活癌调节蛋白基因，同时诱导肿瘤 转型 这将通过定量和定性两方面来实现 肿瘤调节蛋白筛选许多不同的细胞类型， 转化缺陷型和温度敏感型致癌病毒 突变体，或由不同的化学致癌物转化。 蜂窝 将通过首先建立超- 这种蛋白质的结构亚细胞定位，并通过确定是否 肿瘤调节蛋白负责肿瘤特性，如侵袭性， 作为一个模型，正常的侵入性滋养层细胞，这是一个 制造这种蛋白质的少数非肿瘤细胞。

Neoplastic transformation changes the basal concentration of several calcium-binding proteins, calmodulin, S-100, and skin CaBP. The oncodevelopmental calcium-binding protein oncomodulin may also appear in neoplasia, but is different from these others in that it is not found in normal rodent or human adult tissue, and appears in 85% of tumours of diverse origin. It is the long term objective to fit the appearance of oncomodulin into this general perturbation in calcium transport and modulator proteins which must be responsible for some part of the neoplastic phenotype. We must determine whether the expression of the oncomodulin gene is related to the functioning of a particular oncogene, or insertion of a virally borne oncongene into a specific part of the cell genome, or is a general response to neoplastic development. Also of interest is whether transfection of normal cells by DNA from chemically transformed cells activates the oncomodulin gene while inducing neoplastic transformation. This will be achieved by both quantitative and qualitative oncomodulin screening of many different cell types infected by competent, transformation-defective, and temperature sensitive oncogenic viral mutants, or transformed by different chemical carcinogens. The cellular function of oncomodulin will be sought by establishing first the ultra- structural subcellular location of this protein, and by determining whether oncomodulin is responsible for tumour properties, such as invasiveness, by using as a model the normally invasive trophoblast cell, which is one of the few non-neoplastic cells that make this protein.