Further development of an African swine fever virus live attenuated vaccine

非洲猪瘟病毒（ASFV）引起家猪的严重疾病，可导致高达100%的死亡率，并具有严重的社会经济影响。ASFV在大多数撒哈拉以南非洲国家和撒丁岛流行。自非洲猪瘟于2007年传入格鲁吉亚以来，该疾病已在俄罗斯联邦迅速传播，进一步传播的风险增加。缺乏疫苗限制了疾病控制的选择。弱毒ASFV株OURT 88/3在猪中诱导良好水平的保护以抵抗强毒病毒的致死性攻击。由于缺乏合适的细胞系来繁殖病毒，该毒株作为疫苗的商业开发受到阻碍。我们现在已经鉴定了猪巨噬细胞系可以被OURT 88/3毒株感染，并且在这些细胞中生长的病毒仍然可以在猪中诱导保护。我们将研究该细胞系用于ASFV疫苗株生产和诊断的用途。我们还将确定商业开发的合作伙伴

African swine fever virus (ASFV) causes a severe disease of domestic pigs that can result in up to 100% mortality and has severe socio-economic impacts. ASFV is endemic in most sub-Saharan African countries and Sardinia. Since introduction of ASF in 2007 to Georgia, the disease has rapidly spread through the Russian Federation and the risk of further spread has increased. The lack of a vaccine limits options for disease control. Attenuated ASFV strain OURT88/3 induces good levels of protection in pigs against lethal challenge with virulent virus. Commercial development of this strain as a vaccine has been hampered by the lack of a suitable cell line to propagate the virus. We have now identified a pig macrophage cell line can be infected by OURT88/3 strain and the virus grown in these cells can still induce protection in pigs. We will investigate the use of this cell line for ASFV vaccine strain production and diagnosis. We will also identify partners for commercial development