CHEMISTRY OF TUMORICIDAL MACROPHAGE SURFACE ANTIGENS

Mac-1与补体受体类型相关的基础 将研究三个（CR 3）功能。 假设Mac-1是 将通过确定纯化的Mac-1是否可以与CR 3相互作用来测试CR 3。 用C3 bi包覆的颗粒。 进一步的MAb结合不同的地形 Mac-1的细胞外和细胞内结构域将被 制备 C3 bi结合位点的位置将通过 比较单克隆抗体的抑制作用与其对亚基的特异性 （α或β）和Mac-1的蛋白水解片段。 Mac-1的绑定 将测试碎片与C3 bi包被的颗粒的结合。 Mac-1是否是 将研究回收的受体。 稳态比 将测定细胞外/细胞内Mac-1。 Mac-1的变化 将检查配体结合后发生的修饰的结构。 Mac-1在膜中的取向和排列，以及 细胞内、细胞外和膜内结构域将被 研究了 蛋白水解片段的位置将通过以下方法确定： 与MAb对不同地形区域的矢量标记和反应性 以及细胞外和细胞内结构域。 Mac-1与抗原LFA-1同源性的结构基础 与T淋巴细胞介导的杀伤相关，将进一步 研究了 Mac-1将被纯化，并将片段进行氨基酸分析。 测序 N-末端测序表明，α亚基是 33%同源。 （简体中文）

The basis for the association between Mac-1 and complement receptor type three (CR3) function will be investigated. The hypothesis that Mac-1 is the CR3 will be tested by determining whether purified Mac-1 can interact with C3bi-coated particles. Further MAb binding to different topographic regions and extracellular and intracellular domains of Mac-1 will be prepared. The location of the C3bi binding site will be probed by comparing the inhibitory effect of MAb with their specificity for subunits (alpha or beta) and proteolytic fragments of Mac-1. Binding of Mac-1 fragments to C3bi-coated particles will be tested. Whether Mac-1 is a receptor which is recycled will be investigated. The steady state ratio of extracellular/intracellular Mac-1 will be determined. Changes in Mac-1 structure of modification occurring after ligand binding will be examined. The orientation and arrangement of Mac-1 in the membrane, and the size of intracellular, extracellular, and intramembranous domains will be investigated. The location of proteolytic fragments will be determined by vectorial labeling and reactivity with MAb to different topographic regions and extracellular and intracellular domains. The structural basis of the homology between Mac-1 and LFA-1, an antigen associated with T lymphoctye-mediated killing, will be further investigated. Mac-1 will be purified and fragments subjected to amino acid sequencing. N-terminal sequencing has shown that the alpha subunits are 33% homologous. (CS)