13TSB_AgriFood: Optimising the delivery of superior genetics through advanced genomic selection of bovine embryos

这项建议是运输及科技局现有两项资助计划的自然延伸，但对两所合作学术机构来说，是一项重大的扩展（即TS/101069“应用先进育种技术扩增和分配牛遗传基因以提高生产效率和可持续性”，涉及诺丁汉大学）和TS/J 003182/1 [猪体外受精和遗传学：一条通往全球可持续发展的道路，涉及肯特大学]），与商业伙伴一起开发先进的牛育种技术（即Paragon/XLVets，Cogent Breeding Ltd和Illumina Inc）将允许销售经过完整基因组评估的胚胎（包括性别鉴定），以便在尽可能早的发育阶段估计后代具有商业重要性的性状的育种价值。本项目将应用先进的育种技术，（体内和体外）植入前牛胚胎，从中进行活检以使用单核苷酸多态性阵列（SNP芯片）询问其基因组组成。目前，对奶牛和肉牛进行基因组筛选是可能的，但对选择的反应受到等待进行基因组选择的小牛妊娠的阻碍。然而，牛胚胎的筛选将通过提前选择时间和减少不需要的不适当性别的小牛的“浪费”来优化上级遗传基因的传递和扩增。因此，该项目将把联合收割机先进的胚胎育种技术与最先进的基因组筛选（所谓的植入前遗传学诊断）和核绘图（将亲本DNA信息与后代的基因组信息结合起来，以提供更多的遗传细节）相结合。它将制定以下策略：（i）使用性别精液进行最佳牛胚胎培养，（ii）胚胎活检以提取少量卵裂球用于基因组分析，（iii）从多重置换扩增产物中绘制SNP基因型数据，以及（iv）提高冷冻解冻胚胎的妊娠建立。通过对小牛进行基因组分析，将证实新技术可用于更有效地向种猪群提供上级遗传学的原理证明。重要的是，该项目旨在开发和使用独特且极具市场价值的产品（即经遗传评估的性别鉴定胚胎），以低成本向全球市场提供上级遗传学，以高福利和生物安全的方式。这将为英国相关商业公司以及英国养牛业的乳制品和牛肉部门带来巨大的经济利益;并确保英国保持技术创新的前沿，保持其相对于目前位于欧洲、北美和南美的大型养殖公司的竞争优势。

This proposal is a natural but significant extension of two existing TSB funded projects involving the two collaborating academic institutions (i.e. TS/101069 ['Applying advanced breeding technologies to amplify and distribute bovine genetics to increase production efficiency and sustainability', involving the University of Nottingham], and TS/J003182/1 [Pig IVF and genetics: a route to global sustainability, involving the University of Kent]) to develop advanced breeding technologies in cattle with commercial partners (i.e. Paragon/XLVets, Cogent Breeding Ltd and Illumina Inc) that will allow the marketing of embryos that have undergone a complete genomic evaluation (including sexing) in order to estimate the breeding value of progeny for traits of commercial importance at the earliest possible stage of development.This project will apply advanced breeding technologies to produce (both in-vivo and in-vitro) pre-implantation bovine embryos from which biopsies will be taken to interrogate their genomic makeup using single nucleotide polymorphism arrays (SNP chips). It is currently possible to screen dairy and beef cattle genomically, but responses to selection are impeded by waiting for the gestation of the calf on which genomic selection is performed. The screening of bovine embryos, however, would optimise the delivery and amplification of superior genetics by advancing the time of selection and reducing "wastage" of unwanted calves of inappropriate sex. This project will, therefore, combine advanced embryo breeding technologies with state-of-the-art genomic screening (so called pre-implantation genetic diagnosis), and karyomapping (combining parental DNA information with the offspring's genomic information to provide more genetic detail). It will develop strategies for: (i) optimal bovine embryo culture using sexed semen, (ii) embryo biopsy in order to extract a small number of blastomeres for genomic analysis, (iii) karyomapping SNP genotype data from Multiple Displacement Amplification products and (iv) ehnancing pregnancy establishment of frozen-thawed embryos. Proof of principle that the new technologies can be used to deliver superior genetics more efficiently to the breeding herd will be confirmed by conducting genomic analyses of calves.Importantly, this project is geared towards the development and use of a unique and highly marketable product (i.e. genetically evaluated sexed embryos) that will deliver superior genetics to the global market at low cost, and in a high-welfare and bio-secure manner. This will have huge economic benefits for the UK based commercial companies concerned, and for both the dairy and beef sectors of the UK cattle industry; and ensure that the UK remains at the cutting edge of technical innovation retaining its competitive advantage over larger breeding companies currently situated in Europe, North and South America.