ANALYSIS OF HUMAN NK FUNCTION

我们的研究衍生出了能增强或抑制 人NK细胞克隆株的裂解活性这些抗体， 来自与NK克隆免疫的小鼠的脾细胞的融合， 在杀伤细胞水平上影响裂解。其中两种抗体 检测I类MHC基因产物和另外两个检测II类MHC基因 产品；它们显然在其他反类别I或类别II中是独一无二的 抗体对NK功能无此影响。这表明MHC 基因产物可能以某种中心方式参与NK功能 作为原始的受体结构。它进一步提出了具体的 质疑为什么这四种抗体或他们检测到的表位是这样的 独一无二。这些发现与细胞表面的一个模型一致 MHC糖蛋白等分子可能在体内发挥功能作用 不同细胞通过其不同结构域的生理学 分子。我们的计划是研究这种可能性，使用 具有良好特性的人类NK克隆和一组抗I类、抗类 II，以及检测不同决定因素的抗LFA-1单抗 这些分子对NK功能有不同的影响。(CS)

Our research derived monoclonal antibodies which either enhance or inhibit the lytic activity of a cloned iine of human NK cells. These antibodies, derived from fusions with spleen cells of mice immunized with the NK clone, affect lysis at the level of the killer cell. Two of these antibodies detect class I MHC gene products and two others detect class II MHC gene products; they are apparently unique in that other anticlass I or class II antibodies have no such effects on NK function. This suggests that MHC gene products are involved in some central way in NK function, perhaps serving as primitive receptor structures. It further raises the specific question why these four antibodies, or the epitopes they detect, are so unique. These findings are consistent with a model that a cell surface molecule such as an MHC glycoprotein may exert its functional role in the physiology of different cells via different structural domains of that molecule. It is our plan to examine this possibility using well-characterized human NK clones and a panel of anticlass I, anticlass II, and anti-LFA-1 monoclonal antibodies that detect different determinants on these molecules and which have different effects on NK function. (CS)