REGULATION OF IA GENE EXPRESSION

我们的研究将包括研究小鼠Ia的组织分布 基因表达，通过分析Ia特异性信使RNA的存在， 组织和细胞系。 我们将使用来自四个克隆Ia的DNA探针， 基因，A-α，A-β，E-α和E-β，与三个 灵敏的RNA检测、斑点印迹、北方印迹和S1 核酸酶作图， 严格评估Ia基因表达的组织能力，以及 它们的Ia基因诱导能力。 S1 核酸酶图谱也将是 用于确定Ia mRNA是否存在任何替代加工。 我们 移植将决定非骨髓来源的细胞的能力， 通过产生辐射嵌合体表达Ia基因。 的特点 在组织和细胞系包括巨噬细胞中的Ia基因诱导， 树突细胞和T细胞将使用已知的诱导剂进行评估 包括γ干扰素。 我们打算严格测试最近的一个 假设Ia抗原在移植组织中被诱导到其他细胞上， 而不是“过客白细胞”作为同种异体移植反应的结果。 最后，我们将研究Ia基因的诱导机制， 将Ia基因克隆到不同的细胞类型中， 表情（MB）

Our research will include studying the tissue distribution of murine Ia gene expression by analyzing the presence of Ia-specific messenger RNAs in tissues and cell lines. We will use DNA probes derived from four cloned Ia genes, A-alpha, A-beta, E-alpha, and E-beta, in combination with three sensitive RNA assays, dot blots, Northern blots, and S1 nuclease mapping, to critically evaluate the tissues capable of Ia gene expression as well as their capacity for Ia gene induction. S1 nuclease mapping will aIso be used to determine if there is any alternative processing of Ia mRNA. Our investigstion will determine the ability of nonbone marrow-derived cells to express Ia genes by producing radiation chimeras. The characteristics of Ia gene induction in both tissues and cell lines including macrophages, dendritic cells, and T cells will be evaluated using known inducing agents including gamma interferon. We intend to critically test a recent hypothesis that Ia antigens are induced in grafted tissue on cells other than "passenger leukocytes" as a consequence of the allograft reaction. Finally, we will study the mechanism of Ia gene induction by transfecting cloned Ia genes into different cell types and selecting for inducible expression. (MB)