LYMPHOKINES, CELLULAR IMMUNITY, DELAYED HYPERSENSITIVITY

主要目的是确定以下各项之间的相互关系：(1) 细胞免疫(CMI)；(2)体内释放迁移抑制 因子(MIF)和/或干扰素-γ(干扰素-γ)；以及(3)延迟 过敏症。这些研究将在两种类型的自交系中进行 小鼠：(1)对白色念珠菌和牛分枝杆菌耐药的小鼠 卡介苗(BCG)，高反应者(体内释放两种淋巴因子，MIF 和干扰素)，以及迟发性超敏反应的良好反应；和(2) 容易感染白色念珠菌，体内MIF反应率低， 以及迟发性超敏反应的不良反应。将计划进行实验 并运行以分离参与形成或 上述三种免疫现象的发生发展。这个 编码MIF或相关因素的信使RNA组分 细胞介导的免疫将被提纯。将制备cDNA，并将其 在大肠杆菌中通过分子克隆分离到负责基因(S)。这样的克隆 然后将用于制作MIF的因素(S)引起 细胞免疫。然后对这些提纯因子进行分析 它们能够增强敏感小鼠品系的CMI。这些细胞 负责淋巴因子的形成和发展 细胞介导的免疫将被分离和鉴定。也就是 增强或抑制CMI的胸腺肽将被测定，并 我们将分析它们所影响的特定细胞电路。(Hf)

The primary aim is to determine the interrelationship between: (1) cell-mediated immunity (CMI); (2) in vivo release of migration inhibitory factor (MIF) and/or interferon-gamma (IFN-gamma); and (3) delayed hypersensitivity. The studies will be carried out in two types of inbred mice: (1) those resistant to Candida albicans and Mycobacterium bovis (BCG), high responders (in the in vivo release of the two lymphokines, MIF and IFN), and good reactors in delayed hypersensitivity; and (2) those susceptible to infection with C. albicans, low responders in in vivo MIF, and poor reactors in delayed hypersensitivity. Experiments will be planned and run to isolate the messenger RNAs involved in the formation or development of the three aforementioned immunologic phenomena. The messenger RNA fractions coding for MIF or a related factor causing cell-mediated immunity will be purified. The cDNA will be prepared and the responsible gene(s) isolated by molecular cloning in E. coli. Such clones will then be used for the production of MIF of the factor(s) causing cell-mediated immunity. These purified factors will then be analyzed for their capacity to enhance CMI in the susceptible murine strains. The cells responsible for the formation of the lymphokines and for the development of cell-mediated immunity will be separated and characterized. Also the thymic peptides that enhance or suppress CMI will be determined and the particular cellular circuits that they effect will be analyzed. (HF)