ANTIBODY TO GLUCOSYLTRANSFERASE IN ADULTS AND CHILDREN

唾液对口服抗原的免疫反应可能调节数量和 定植于口腔的微生物的类型。这些响应可能 因暴露于肠道内的抗原或暴露于淋巴炎而被诱发 口腔中的组织，例如，在小唾液腺中。一个 对婴幼儿免疫功能发育的认识 年幼的孩子，以及儿童第一次出现的年龄的身份虚构 应对口腔链球菌，如唾液链球菌，广为人知的时尚。 糖基转移酶是这些链球菌的胞外产物 合成大小的葡聚糖，其中一些被认为与 变形链球菌的致龋性。为此，本调查提出 为了测量IgA抗体水平的纵向发展， 唾液链球菌、血链球菌和变形链球菌的葡萄糖转移酶抗原 在婴儿和五岁以下儿童的全唾液中。这个 这些口腔链球菌的存在也将通过选择性的 斑块或口腔粘膜的电镀，以识别最初的外观 这些链球菌中。这些微生物的鉴定将是 与抵抗性抗原的抗体水平相关。这个 儿童唾液抗体亚型分布，尤其是唾液抗体 六个月以下的儿童将接受高压调查 高效液相色谱法和酶联免疫吸附试验(ELISA)。这个 2、4和6月龄口腔脊髓灰质炎婴儿的抗原性挑战 疫苗和注射用百白破疫苗代表了一种有用的探索模式 口腔功能不全形成过程中的免疫反应 空洞。因此，一个纵向研究的外观和变化在 唾液中对脊髓灰质炎病毒1型、2型和3型以及破伤风的抗体水平 毒素类药物将在五岁以下的儿童中使用。最后， 在口腔中引发局部免疫反应的能力将是 通过将破伤风类毒素应用于嘴唇粘膜进行调查。 用酶联免疫吸附试验检测嘴唇小腺分泌物的反应。 这些数据应该揭示了局部抗原刺激的可能性 口腔和未来免疫方案的这一路线的使用。

The salivary immune response to oral antigens may modulate the number and type of microorganisms which colonize the oral cavity. These responses may be elicited by exposure to antigen in the gut or by exposure of lymphtic tissue in the oral cavity, for example, in minor salivary glands. An understanding of the development of immunocompetence in the infant and young child, and an identifiction of the ages at which children first respond to oral streptococci, such as S. salivrius, knowlegeable fashion. Glucosyltransferases are extracellular products of these streptococci which synethesize glucans, some of which have been implicated in the cariogenicity of S. mutans. To this end, the present investigtion proposes to measure the longitudinal development of IgA antibody levels to glucosyltransferase antigens from S. salivrius, S. sanguis, and S. mutans in whole salivas of infants and children up to five years of age. The presence of these oral streptococci will also be ascertained by selective plating of plaque or oral mucosa in order to identify the irst appearance of these streptococci. The identification of these microorganisms will be correlated with antibody levels to the repective antigens. The distribution of isotypes of salivary antibody, especially in salivas from children younger than six months, will be investigted by high pressure liquid chromatography and enzyme-linked immunosorben assay (ELISA). The antigenic challenge of infants at 2,4 and 6 months of age with oral polio vaccine and parenteral DPT vaccine represents a useful model for exploring immune responses during the development of immuocompetance in the oral cavity. Thus, a longitudinal study of the appearance and changes in salivary antibody levels to poliovirus typs 1, 2 and 3 and to tetanus toxoid will be conducted in children up to five yers of age. Finally, the ability to elicit a local immune response in the oral cavity will be investigated by application of tetanus toxoid to the labial mucosae. Responses will be measured by ELISA in labial minor gland secretions. These data should reveal the potential for local antigenic stimulation in the oral cavity and the use of this route for future immunization protocols.