PROPERTIES OF LIPOTEICHOIC ACIDS--CARIOGENIC BACTERIA

变形链球菌在龋病中起重要作用。 长期协议， 致龋菌和非致龋菌可能具有不同的理化性质， 这些特性可导致不同的免疫原性特征。 我们 关于生物体的知识现在已经发展到一个阶段， 生物学研究的基本单位是分子而不是 细胞，从而将生物学转变为分子或化学科学。 的 大分子所表现出的生物学特性不仅是由于它们 化学成分和结构，而且还与它们的物理和 构象性质 提取物，免疫特性， LTA的生物学特性和生物合成已经被广泛地 研究了 对此，知之甚少。 长期协议的物理化学性质。 因此，它是总体具体的 该项目的目的是获得关于物理化学的确切数据， LTA的参数。 将LTA和脱酰部分从 致龋链球菌变形链球菌Ingbritt和BHT。化脓，和 S. faecalis ATTCC 8790从细胞和细胞外来源，将是 从溶液中检测，使用小角X射线散射方法， 沉降分析和粘度测定。 这些测量将提供 关于其形状和大小的信息。 例如分子量、半径 分子的旋转及其横截面，单位长度的质量， 长期目标是 比较获得的LTA的物理和构象性质 从各种致病性和非致病性细菌，并将这些 发现其致病性和免疫原性。

Streptococcus mutans plays a significant role in dental caries. LTAs from cariogenic and non cariogenic bacteria may have different physico-chemical properties which can lead to different immunogenic characteristics. Our knowledge of living organisms has now reached a state of development where the fundamental unit of biological study is the molecule rather than the cell, thus shifting biology to a molecular or chemical science. The biological properties exhibited by macromolecules are not only due to their chemical composition and configuration but also to their physical and conformational properties. The extraction, immunological properties, biological properties and biosynthesis of LTAs have been extensively investigated. In contrast to this, little is known concerning the physico-chemical nature of the LTAs. Therefore, it is the overall specific aim of this project to obtain definitive data on the physico-chemical parameters of the LTAs. The LTAs and deacylated moiety extracted from cariogenic S. mutans Ingbritt and BHT from non-cariogenic S. pyogenes, and S. faecalis ATTCC 8790 from cellular and extracellular sources, will be examined from solution, using the methods of small angle x-ray scattering, sedimentation analysis and viscometry. These measurements will provide information on their shape and size. e.g. molecular weight, radius of gyration of the molecule and its cross-section, mass per unit length, hydrodynamic volume, persistence length etc. Long term objective is to compare the physical and conformational properties of the LTAs obtained from various pathogenic and non-pathogenic bacteria and to correlate these findings to their pathogenicity and immunogenicity.