SALIVA FORMATION STUDIED BY MICROPROBE ANALYSIS

这项研究的最终目的是了解细胞机制 唾液腺中的电解质和液体运输。 一个相关 我们的目标是了解这些运输过程是如何影响在囊性 纤维化 具体目标是：1.用电子探针 Na、Mg、P、S、Cl、K和Ca元素浓度测量技术 在基底和顶端细胞质、分泌颗粒、细胞核、线粒体 非刺激性大鼠腮腺细胞间隙和管腔的变化 腺泡。 2.用电子探针技术测定上述各参数 所列细胞内和细胞外的元素浓度 用胆碱能和α诱导流涎后的空间， β-肾上腺素能促分泌素。 3.使用测得的钠、氯等 以上列出的元素浓度，以测试跨细胞和 细胞旁电解质的变化，以及伴随的水通量， 预测溶质-溶剂耦合假设水流在 上皮组织 4.进行类似的元素分析， 腮腺的闰管和纹管，以确定 如果有可观的电解质或水流过这些 结构. 5.为了在腮腺中进行类似的分析， 利血平动物，以确定如何这些电解质和水的运输 机制受到影响。 这些发现将有助于我们 了解唾液腺生理学和运输的变化， 利血平化大鼠--人类囊性纤维化模型。

The ultimate goal of this research is to understand the cellular mechanisms underlying electrolyte and fluid transport in salivary glands. A related goal is to understand how these transport processes are affected in cystic fibrosis. The specific aims are: 1. To use the electron microprobe technique to measure elemental concentrations of Na, Mg, P, S, Cl, K and Ca in basal and apical cytoplasm, secretory granules, nuclei, mitochondria, lateral intercellular spaces and lumen of nonstimulated rat parotid gland acini. 2. To use the electron microprobe technique to measure the above elemental concentrations in the listed intracellular and extracellular spaces following induction of salivation with cholinergic and Alpha and Beta-adrenergic secretagogues. 3. To use the measured Na, Cl and other elemental concentrations listed above to test for the transcellular and paracellular electrolyte changes, and accompanying water fluxes, that are predicted by the solute-solvent coupled hypothesis for water flow in epithelial tissues. 4. To perform similar elemental analyses in intercalated and striated ducts from parotid glands in order to determine if there are appreciable electrolyte or water fluxes across these structures. 5. To perform similar analyses in parotid glands from reserpinized animals to determine how these electrolyte and water transport mechanisms are affected. These findings will contribute to our understanding of salivary gland physiology and of transport changes in the reserpinized rat--a model for cystic fibrosis in man.